Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | TTP, dATP, TTP/GDP, TTP/ATP, and TTP/dATP, 1. TTP bound at the S-site, 2. dATP bound at the S-site, 3. TTP bound at the S-site and GDP at the C-site, 4. TTP bound at the S-site and ATP at the A-site, and 5. TTP bound at the S-site and dATP at the A-site | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
subunit RR1 in complex with TTP, dATP, TTP/GDP, TTP/ATP, and TTP/dATP, 1. TTP bound at the S-site, 2. dATP bound at the S-site, 3. TTP bound at the S-site and GDP at the C-site, 4. TTP bound at the S-site and ATP at the A-site, and 5. TTP bound at the S-site and dATP at the A-site, X-ray diffraction structure determination and analysis at resolutions of 2.4 A, 2.3 A, 3.2 A, 3.1 A, and 3.1 A, respectively | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D16R | site-directed mutagenesis, the mutant retains 55% of wild-type activity for CDP reduction, and 67% for ADP reduction, it is not inhibited and does not form hexamers at physiologically relevant dATP concentrations | Homo sapiens |
D57N | site-directed mutagenesis, the mutant is not inhibited and does not form hexamers at physiologically relevant dATP concentrations, the mutation of Asp 57 to Asn abolishes the salt-bridge and change the electrostatic environment of the A-site, resulting in loss of allosteric regulation by dATP | Homo sapiens |
H2E | site-directed mutagenesis, the mutant retains 56% of wild-type activity for CDP reduction, and 56% for ADP reduction | Homo sapiens |
Organism | UniProt | Comment | Textmining |
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Homo sapiens | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
More | dATP-induced oligomerization, overview, modeling of the holo-complex | Homo sapiens |
multimer | alphanbetan multi-subunit protein complex consisting of subunit types RR1 and RR2, the alpha or RR1 subunit contains the catalytic C site and two allosteric sites, while the beta or RR2 subunit houses a stable tyrosyl free radical that is transferred some 35 A to the catalytic site to initiate radical-based chemistry on the substrate | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
class I RR | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | RR is regulated transcriptionally and allosterically. RR activity is also controlled by subunit RR2 levels | Homo sapiens |
additional information | structural basis for substrate selection, RR activity is lowest at high dATP concentrations when the hexamer population is high, egulation of RR by dATPinduced oligomerization, overview. RR is regulated transcriptionally and allosterically. RR is further regulated by subunit localization and by its protein inhibitor Sml1 | Homo sapiens |