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Literature summary for 1.17.4.1 extracted from

  • Cotruvo, J.A.; Stubbe, J.
    Escherichia coli class Ib ribonucleotide reductase contains a dimanganese(III)-tyrosyl radical cofactor in vivo (2011), Biochemistry, 50, 1672-1681.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.4.1

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ diferric(III)-tyrosyl radical cofactor Escherichia coli
Mn3+ dimanganese(III)-tyrosyl radical cofactor Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 genes nrdE and nrdF encoding the subunits alpha2 and beta2
-
Escherichia coli GR536
-
 genes nrdE and nrdF encoding the subunits alpha2 and beta2
-

Purification (Commentary)

Purification (Comment) Organism
native NrdF from strain GR536 Escherichia coli

Synonyms

Synonyms Comment Organism
class Ib ribonucleotide reductase
-
 Escherichia coli
class Ib RNR
-
 Escherichia coli
NrdE
-
 Escherichia coli
NrdF
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
diferric(III)-tyrosyl radical cofactor FeIII2-tyrosyl radical cofactor Escherichia coli
dimanganese(III)-tyrosyl radical cofactor MnIII2-tyrosyl radical cofactor Escherichia coli
additional information RNR is active with both FeIII2-tyrosyl radical and MnIII2-tyrosyl radical cofactors in the beta2 subunit, NrdF Escherichia coli

General Information

General Information Comment Organism
physiological function class Ib RNR converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions Escherichia coli