Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dATP | positive effector | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-methoxyphenol | - |
Corynebacterium glutamicum | |
EDTA | - |
Corynebacterium glutamicum | |
Hydroxyurea | - |
Corynebacterium glutamicum | |
additional information | the enzyme is inhibited by radical scavengers | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains only 0.06 mol iron per mol of R2F subunit | Corynebacterium glutamicum | |
Mn2+ | 0.8 mol manganese per mol of R2F subunit, oragnized in a coupled binuclear centre with paramagnetic ground state or in a weakly coupled binuclear centre with therminally populated paramagnetic excites state which appears as binuclear Mn2+, overview | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, R2F subunit, DSD-PAGE | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene nrdF encoding subunit R2F | - |
Corynebacterium glutamicum R163 | - |
gene nrdF encoding subunit R2F | - |
Purification (Comment) | Organism |
---|---|
native R2F subunit by 2',5'-ADP affinity and hydrophobic interaction chromatography, followed by two different steps of gel filtration, an further by anion exchange chromnatography for metallo-cofactor analysis | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
subunit R2F | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CDP as substrate | Corynebacterium glutamicum | ? | - |
? | |
additional information | CDP as substrate | Corynebacterium glutamicum R163 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, R2F subunit, DSD-PAGE | Corynebacterium glutamicum |
More | hybrid holoenzyme, consisting of the small manganese-containing R2F subunit and the large catalytic subunit R1E | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
manganese-ribonucleotide reductase | - |
Corynebacterium glutamicum |
Mn-RNR | - |
Corynebacterium glutamicum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.5 | - |
- |
Corynebacterium glutamicum | 4-methoxyphenol | |
1.7 | - |
- |
Corynebacterium glutamicum | Hydroxyurea | |
2.4 | - |
- |
Corynebacterium glutamicum | EDTA |