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Literature summary for 1.17.4.1 extracted from

  • Voevodskaya, N.; Lendzian, F.; Ehrenberg, A.; Graeslund, A.
    High catalytic activity achieved with a mixed manganese-iron site in protein R2 of Chlamydia ribonucleotide reductase (2007), FEBS Lett., 581, 3351-3355.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Chlamydia trachomatis
DTT required for activity Chlamydia trachomatis

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli Chlamydia trachomatis

Protein Variants

Protein Variants Comment Organism
additional information construction of truncated R1 mutant DELTA1-248 Chlamydia trachomatis

Inhibitors

Inhibitors Comment Organism Structure
Hydroxyurea inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)–Fe(III) state Chlamydia trachomatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme redox states, overview Chlamydia trachomatis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview Chlamydia trachomatis
Mg2+
-
Chlamydia trachomatis
Mn2+ the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview Chlamydia trachomatis
additional information enzyme redox states, overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP + thioredoxin Chlamydia trachomatis
-
2'-deoxyCDP + thioredoxin disulfide + H2O
-
?
ribonucleoside diphosphate + thioredoxin Chlamydia trachomatis
-
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin catalytic mechanism Chlamydia trachomatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248 Chlamydia trachomatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP + thioredoxin
-
Chlamydia trachomatis 2'-deoxyCDP + thioredoxin disulfide + H2O
-
?
ribonucleoside diphosphate + thioredoxin
-
Chlamydia trachomatis 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?
ribonucleoside diphosphate + thioredoxin the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2 Chlamydia trachomatis 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 2 * R1 subunit + 2 * R2 subunit Chlamydia trachomatis

Synonyms

Synonyms Comment Organism
More a class I ribonucleotide reductase Chlamydia trachomatis
RNR
-
Chlamydia trachomatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Chlamydia trachomatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Chlamydia trachomatis

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
Chlamydia trachomatis