Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Chlamydia trachomatis | |
DTT | required for activity | Chlamydia trachomatis |
Cloned (Comment) | Organism |
---|---|
expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli | Chlamydia trachomatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated R1 mutant DELTA1-248 | Chlamydia trachomatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxyurea | inhibition of the enzyme by the 1-electron donor hydroxyurea produces the Mn(III)Fe(III) state | Chlamydia trachomatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme redox states, overview | Chlamydia trachomatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview | Chlamydia trachomatis | |
Mg2+ | - |
Chlamydia trachomatis | |
Mn2+ | the manganese- and iron content of the R2 subunit decides about the enzyme activity, determination of metal contents, overview | Chlamydia trachomatis | |
additional information | enzyme redox states, overview | Chlamydia trachomatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | Chlamydia trachomatis | - |
2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
ribonucleoside diphosphate + thioredoxin | Chlamydia trachomatis | - |
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia trachomatis | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | catalytic mechanism | Chlamydia trachomatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activities of recombinant wild-type R1/R2 and chimeric wild-type R2 with truncated R1 mutant DELTA1-248 | Chlamydia trachomatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? | |
ribonucleoside diphosphate + thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? | |
ribonucleoside diphosphate + thioredoxin | the catalytic reaction in class I RNR involves a long-range electron transfer, coupled to proton transfer, between the substrate binding site in protein R1 and the iron/radical site in protein R2 | Chlamydia trachomatis | 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * R1 subunit + 2 * R2 subunit | Chlamydia trachomatis |
Synonyms | Comment | Organism |
---|---|---|
More | a class I ribonucleotide reductase | Chlamydia trachomatis |
RNR | - |
Chlamydia trachomatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chlamydia trachomatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Chlamydia trachomatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Chlamydia trachomatis |