Literature summary for 1.17.4.1 extracted from

Racay, P.; Kollarova, M.
Purification and partial characterization of Ca2+-dependent ribonucleotide reductase from Streptomyces aureofaciens (1996), Biochem. Mol. Biol. Int., 38, 493-500.

Search for more literature for 1.17.4.1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activity depends on Ca2+	Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60200	-	2 * 60200, dimer appears to dissociate in the absence of Ca2+ into monomers, SDS-PAGE	Kitasatospora aureofaciens
125900	-	5 mM Ca2+, gel filtration	Kitasatospora aureofaciens

Organism

Organism	UniProt	Comment	Textmining
Kitasatospora aureofaciens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
streptomycin, DEAE-Sepharose, Phenyl-Sepharose, Heparin-Sepharose, Sephacryl S-200	Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.1	-	-	Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ribonucleoside diphosphate + reduced thioredoxin	-	Kitasatospora aureofaciens	2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O	-	ir

Subunits

Subunits	Comment	Organism
dimer	2 * 60200, dimer appears to dissociate in the absence of Ca2+ into monomers, SDS-PAGE	Kitasatospora aureofaciens

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Release 2023.1 (January 2023)