General Stability | Organism |
---|---|
enzyme 1 is not stable in potassium phosphate buffer pH 7.5, 10 vol% methanol, ethanol or propanol stabilizes | Pseudomonas putida |
enzyme 2 is most stable in imidazole/HCl buffer, 10 vol% acetone stabilizes | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3'-dithiobis(6-nitrobenzoate) | weak inhibition | Pseudomonas putida | |
HgCl2 | at 0.5 mM complete inhibition of enzyme 1 and enzyme 2 | Pseudomonas putida | |
p-chloromercuribenzoate | complete inhibition of enzyme 1 and enzyme 2 | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
5-hydroxy-1,4-naphthoquinone | enzyme 1 | Pseudomonas putida | |
0.0185 | - |
5-hydroxy-1,4-naphthoquinone | enzyme 2 | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 0.39 atoms per subunit for enzyme 1, 0.04 atoms per subunit for enzyme 2 | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23500 | - |
2 * 23500, enzyme 2, SDS-PAGE | Pseudomonas putida |
25000 | - |
2 * 25000, enzyme 1, SDS-PAGE | Pseudomonas putida |
56000 | - |
enzyme 2, gel filtration | Pseudomonas putida |
59000 | - |
enzyme 1, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-1,4-naphthoquinone + O2 | Pseudomonas putida | - |
3,5-dihydroxy-1,4-naphthoquinone + H2O | - |
? | |
5-hydroxy-1,4-naphthoquinone + O2 | Pseudomonas putida J1 | - |
3,5-dihydroxy-1,4-naphthoquinone + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
two isofunctional enzymes named enzyme 1, present in induced and non-induced cells and enzyme 2, present only in juglone-induced cells | - |
Pseudomonas putida J1 | - |
two isofunctional enzymes named enzyme 1, present in induced and non-induced cells and enzyme 2, present only in juglone-induced cells | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
242 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-naphthoquinone + O2 | - |
Pseudomonas putida | 2-hydroxy-1,4-naphthoquinone + H2O | - |
? | |
1,4-naphthoquinone + O2 | - |
Pseudomonas putida J1 | 2-hydroxy-1,4-naphthoquinone + H2O | - |
? | |
2-chloro-1,4-naphthoquinone + O2 | - |
Pseudomonas putida | 2-chloro-3-hydroxy-1,4-naphthoquinone + H2O | - |
? | |
2-chloro-1,4-naphthoquinone + O2 | - |
Pseudomonas putida J1 | 2-chloro-3-hydroxy-1,4-naphthoquinone + H2O | - |
? | |
5,8-dihydroxy-1,4-naphthoquinone + O2 | - |
Pseudomonas putida | 2,5,8-trihydroxy-1,4-naphthoquinone + H2O | - |
? | |
5,8-dihydroxy-1,4-naphthoquinone + O2 | - |
Pseudomonas putida J1 | 2,5,8-trihydroxy-1,4-naphthoquinone + H2O | - |
? | |
5-hydroxy-1,4-naphthoquinone + O2 | - |
Pseudomonas putida | 3,5-dihydroxy-1,4-naphthoquinone + H2O | - |
? | |
5-hydroxy-1,4-naphthoquinone + O2 | - |
Pseudomonas putida J1 | 3,5-dihydroxy-1,4-naphthoquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * 25000, enzyme 1, SDS-PAGE | Pseudomonas putida |
dimer | 2 * 23500, enzyme 2, SDS-PAGE | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | enzyme 1 and enzyme 2 | Pseudomonas putida |