Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for rational design of flavonoid-type inhibitors against xanthine oxidase useful for the treatment of hyperuricemia, gout, and inflammatory disease states | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
enzme in complex with inhibitor quercetin, X-ray diffraction structure determintion and analysis at 2.0 A resolution | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | natural flavonoids are attractive leads for rational design of preventive and therapeutic xanthine oxidase inhibitors due to their beneficial antioxidant, anti-inflammatory, and antiproliferative activities in addition to their micromolar inhibitory activities toward xanthine oxidase | Bos taurus | |
quercetin | structure analysis of quercetin in complex with the enzyme, overview. The inhibitor adopts a single orientation with its benzopyran moiety sandwiched between Phe 914 and Phe 1009 and ring B pointing toward the solvent channel leading to the molybdenum active center. The favorable steric complementarity of the conjugated three-ring structure of quercetin with the active site and specific hydrogen-bonding interactions of exocyclic hydroxy groups with catalytically relevant residues Arg 880 and Glu 802 correlate well with a previously reported structure-activity relationship of flavonoid inhibitors of xanthine oxidase | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | Bos taurus | - |
xanthine + NADH + H+ | - |
? | |
xanthine + H2O + O2 | Bos taurus | - |
urate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P80457 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Bos taurus | - |
milk | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H2O | - |
Bos taurus | xanthine + NADH + H+ | - |
? | |
xanthine + H2O + O2 | - |
Bos taurus | urate + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bos taurus |