Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.3.2 extracted from

  • Bray, R.C.
    Molybdenum iron-sulfur flavin hydroxylases and related enzymes (1975), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 12, 299-419.
No PubMed abstract available
Search for more literature for 1.17.3.2

Inhibitors

Inhibitors Comment Organism Structure
2,4-Diamino-6-hydroxy-s-triazine
-
 Bos taurus
2,4-dinitrofluorobenzene
-
 Bos taurus
2-Amino-4-hydroxypterine-6-aldehyde
-
 Bos taurus
allopurinol
-
 Bos taurus
arsenite
-
 Bos taurus
cyanide
-
 Bos taurus
cyanide
-
 Gallus gallus
formaldehyde
-
 Bos taurus
guanine
-
 Bos taurus
methanol
-
 Bos taurus
methanol
-
 Gallus gallus
Purine-6-aldehyde
-
 Bos taurus
salicylate
-
 Bos taurus
thiocyanate
-
 Drosophila melanogaster
thiocyanate
-
 Gallus gallus
Urea
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Drosophila melanogaster
additional information
-
 additional information
-
 Bos taurus
0.05
-
 O2 cosubstrate xanthine, pH 8.5 Bos taurus
0.08
-
 O2 cosubstrate xanthine, pH 10.0 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-molybdenum protein Gallus gallus
Iron iron-molybdenum protein Drosophila melanogaster
Iron iron-molybdenum protein Bos taurus
Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Gallus gallus
Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Drosophila melanogaster
Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Bos taurus
Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Gallus gallus
Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Drosophila melanogaster
Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Bos taurus
Molybdenum an iron-molybdenum protein Gallus gallus
Molybdenum an iron-molybdenum protein Drosophila melanogaster
Molybdenum an iron-molybdenum protein Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Gallus gallus
additional information
-
-
 Bos taurus
120000
-
 2 * 120000 Gallus gallus
150000
-
 2 * 150000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Drosophila melanogaster
-
-
-
Gallus gallus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information
-
 Gallus gallus
additional information
-
 Drosophila melanogaster
additional information
-
 Bos taurus
proteolytic modification
-
 Gallus gallus
proteolytic modification
-
 Drosophila melanogaster
proteolytic modification
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
xanthine + H2O + O2 = urate + H2O2 mechanism Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-
liver
-
 Bos taurus
-
milk
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4 5.1
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-2-hydroxypurine + H2O + O2
-
 Bos taurus 1-methyl-2-hydroxy-7,9-dihydropurin-8-one + H2O2
-
 ?
1-methylxanthine + H2O + O2
-
 Bos taurus 1-methylurate + H2O2
-
 ?
3-methylhypoxanthine + H2O + O2
-
 Bos taurus 3-methylxanthine + H2O2
-
 ?
6,8-dihydroxypurine + H2O + O2
-
 Bos taurus ?
-
 ?
6-cyanopurine + H2O + O2
-
 Bos taurus 6-cyano-7,9-dihydropurine-8-one
-
 ?
6-thioxanthine + H2O + O2
-
 Bos taurus 6-thiourate + H2O2
-
 ?
acetaldehyde + H2O + O2
-
 Bos taurus acetic acid + H2O2
-
 ?
adenine + H2O + O2
-
 Bos taurus 6-amino-7,9-dihydropurin-8-one + H2O2
-
 ?
allopurinol + H2O + O2
-
 Bos taurus ?
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Gallus gallus urate + 2 H2O2
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Drosophila melanogaster urate + 2 H2O2
-
 ?
hypoxanthine + 2 H2O + 2 O2
-
 Bos taurus urate + 2 H2O2
-
 ?
N1-methylnicotinamide + H2O + O2
-
 Bos taurus ?
-
 ?
NADH + H2O + O2
-
 Bos taurus NAD+ + H2O2
-
 ?
pteridine + H2O + O2 and derivatives, e.g.: 4-amino-7-hydroxy pteridine, 4-hydroxy-7-azapteridine Bos taurus ?
-
 ?
purine + H2O + O2 purine and derivatives Bos taurus 7,9-dihydropurin-8-one + H2O2
-
 ?
pyrimidine derivatives + H2O + O2 e.g. 2-hydroxypyrimidine Bos taurus ?
-
 ?
pyrimidine derivatives + H2O + O2 6-hydroxy-6-aminepyrimidine Bos taurus ?
-
 ?
pyrimidine derivatives + H2O + O2 7-hydroxy-(1,2,5)-thiadiazolo(3,4-d)-pyrimidine Gallus gallus ?
-
 ?
pyrimidine derivatives + H2O + O2 7-hydroxy-(1,2,5)-thiadiazolo(3,4-d)-pyrimidine Drosophila melanogaster ?
-
 ?
salicylaldehyde + H2O + O2
-
 Bos taurus salicylic acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor methylene blue Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 electron acceptor NAD+ Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 electron acceptor O2 Gallus gallus uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor O2 Drosophila melanogaster uric acid + H2O2
-
 ?
xanthine + H2O + O2 electron acceptor O2 Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 electron acceptor quinones Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 specificity for electron acceptor is low Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 enzyme also oxidizes hypoxanthine, some other purines, pterines and aldehydes, i.e. possesses the activity of EC 1.2.3.1, probably acts on the hydrated derivatives of these substrates Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 low specificity to substrate Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 electron acceptor ferricyanide Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?

Subunits

Subunits Comment Organism
dimer 2 * 150000, SDS-PAGE Bos taurus
dimer 2 * 130000-140000 Drosophila melanogaster
dimer 2 * 120000 Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.2
-
 xanthine pH 8.2, 23.5°C Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Gallus gallus
FAD flavoprotein Drosophila melanogaster
FAD flavoprotein Bos taurus
FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Gallus gallus
FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Drosophila melanogaster
FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Bos taurus