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Literature summary for 1.17.2.3 extracted from

  • Yagi, T.
    Formate: cytochrome oxidoreductase of Desulfovibrio vulgaris (1969), J. Biochem., 66, 473-478.
    View publication on PubMed
Search for more literature for 1.17.2.3

Inhibitors

Inhibitors Comment Organism Structure
KCN
-
 Desulfovibrio vulgaris
additional information not: p-chloromercuribenzoate, idodoacetamide, sodium hypophosphite, EDTA or o-phenanthroline Desulfovibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
 formate
-
 Desulfovibrio vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 MW of cytochrome c-553: 6500, gel filtration Desulfovibrio vulgaris

Organism

Organism UniProt Comment Textmining
Desulfovibrio vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Desulfovibrio vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Desulfovibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + ferricytochrome c-553 yeast cytochrome c, ferricyanide and phenazine methosulfate can act as acceptors, not: NAD+, NADP+, FAD, FMN, cytochrome c3 Desulfovibrio vulgaris CO2 + ferrocytochrome c-553
-
 ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Desulfovibrio vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 with K3Fe(CN)6 as acceptor Desulfovibrio vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 pH 4.5: about 40% of activity maximum, pH 10: about 10% of activity maximum Desulfovibrio vulgaris