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Literature summary for 1.17.1.9 extracted from

  • Takacs, M.; Makhlynets, O.V.; Tolbert, P.L.; Korendovych, I.V.
    Secretion of functional formate dehydrogenase in Pichia pastoris (2017), Protein Eng. Des. Sel., 30, 381-386 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Application

Application Comment Organism
biofuel production NAD+-dependent formate dehydrogenase is capable of the electrochemical reduction of carbon dioxide into formate, which can be ultimately converted to methanol. Enzyme secretion of formate dehydrogenase by yeast is a promising method for creating multi-enzyme devices for biofuel production [Candida] boidinii

Cloned(Commentary)

Cloned (Comment) Organism
a tagged, functional enzyme can be continuously secreted by Pichia pastoris. The protein can be easily separated from the growth media using Ni-NTA resin and the activity of the enzyme remains constant even after prolonged growth at 29°C [Candida] boidinii
expressed in Escherichia coli BL21(DE3) cells and Pichia pastoris GS115 cells [Candida] boidinii

General Stability

General Stability Organism
the enzyme activity remains constant after prolonged growth at 29°C [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-
[Candida] boidinii O13437
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Candida] boidinii
Ni-NTA column chromatography and Q Sepharose column chromatography [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE [Candida] boidinii

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii
NAD+-dependent formate dehydrogenase
-
 [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii