Application | Comment | Organism |
---|---|---|
drug development | construction of bifunctional formate dehydrogenase and leucine dehydrogenase enzymatic complex for efficient cofactor regeneration and L-tert leucine biotransformation. L-tert leucine is a widely used chiral building block in many asymmetric reactions for the synthesis of anti-tumor and anti-HIV drugs | [Candida] boidinii |
Cloned (Comment) | Organism |
---|---|
a series of bifunctional enzyme complexes are produced by fusing leucine dehydrogenase and formate dehydrogenase with different peptide linkers, which are expressed in Escherichia coli, purified, and exhibit varied parental enzyme activities and varied L-tert leucine catalytic efficiency. The enzymatic reaction system for L-tert-leucine production and cofactor regeneration with suitable peptide linker is potentially more excellent than free enzymes with lower labor-cost on purification, better thermal stability and higher catalytic efficiency compared with the free coupling of parental enzymes | [Candida] boidinii |
expressed in Escherichia coli BL21(DE3) cells | [Candida] boidinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-S3-L | [Candida] boidinii | |
0.037 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-R1-L | [Candida] boidinii | |
0.039 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-DL-L | [Candida] boidinii | |
0.045 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-R3-L | [Candida] boidinii | |
0.051 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-R2-L | [Candida] boidinii | |
0.052 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-S2-L | [Candida] boidinii | |
0.066 | - |
NAD+ | pH 7.5, 30°C, fusion enzyme F-S1-L | [Candida] boidinii | |
0.623 | - |
NAD+ | pH 7.5, 30°C, wild-type enzyme | [Candida] boidinii | |
0.62322 | - |
NAD+ | at pH 7.5 and 30°C | [Candida] boidinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | [Candida] boidinii | - |
CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] boidinii | O13437 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
[Candida] boidinii |
HisTrap column chromatography | [Candida] boidinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH I | - |
[Candida] boidinii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | [Candida] boidinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | [Candida] boidinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] boidinii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
NAD+ | at pH 7.5 and 30°C | [Candida] boidinii |