Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Thiobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Thiobacillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADH | Thiobacillus sp. | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | formate + NAD+ | - |
r | |
CO2 + NADH | Thiobacillus sp. KNK65MA | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | formate + NAD+ | - |
r | |
formate + NAD+ | Thiobacillus sp. | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | CO2 + NADH + H+ | - |
r | |
formate + NAD+ | Thiobacillus sp. KNK65MA | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | CO2 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus sp. | - |
- |
- |
Thiobacillus sp. KNK65MA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Thiobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADH | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | Thiobacillus sp. | formate + NAD+ | - |
r | |
CO2 + NADH | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | Thiobacillus sp. KNK65MA | formate + NAD+ | - |
r | |
formate + NAD+ | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | Thiobacillus sp. | CO2 + NADH + H+ | - |
r | |
formate + NAD+ | the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate | Thiobacillus sp. KNK65MA | CO2 + NADH + H+ | - |
r | |
additional information | - |
Thiobacillus sp. | ? | - |
? | |
additional information | - |
Thiobacillus sp. KNK65MA | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Thiobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Thiobacillus sp. |
NAD-dependent formate dehydrogenase | - |
Thiobacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
formate | at pH 7.0 and 25°C | Thiobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
for CO2 reduction | Thiobacillus sp. |
6.5 | - |
for formate oxidation | Thiobacillus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thiobacillus sp. |