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Literature summary for 1.17.1.9 extracted from

  • Choe, H.; Joo, J.C.; Cho, D.H.; Kim, M.H.; Lee, S.H.; Jung, K.D.; Kim, Y.H.
    Efficient CO2-reducing activity of NAD-dependent formate dehydrogenase from Thiobacillus sp. KNK65MA for formate production from CO2 gas (2014), PLoS ONE, 9, e103111.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(lambdaDE3) cells Thiobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Thiobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CO2 + NADH Thiobacillus sp. the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate formate + NAD+
-
r
CO2 + NADH Thiobacillus sp. KNK65MA the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate formate + NAD+
-
r
formate + NAD+ Thiobacillus sp. the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate CO2 + NADH + H+
-
r
formate + NAD+ Thiobacillus sp. KNK65MA the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate CO2 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Thiobacillus sp.
-
-
-
Thiobacillus sp. KNK65MA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Thiobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CO2 + NADH the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate Thiobacillus sp. formate + NAD+
-
r
CO2 + NADH the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate Thiobacillus sp. KNK65MA formate + NAD+
-
r
formate + NAD+ the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate Thiobacillus sp. CO2 + NADH + H+
-
r
formate + NAD+ the enzyme exhibits a dramatic preference for the CO2 reduction reaction over the oxidation reaction of formate Thiobacillus sp. KNK65MA CO2 + NADH + H+
-
r
additional information
-
Thiobacillus sp. ?
-
?
additional information
-
Thiobacillus sp. KNK65MA ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Thiobacillus sp.

Synonyms

Synonyms Comment Organism
FDH
-
Thiobacillus sp.
NAD-dependent formate dehydrogenase
-
Thiobacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.77
-
formate at pH 7.0 and 25°C Thiobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
for CO2 reduction Thiobacillus sp.
6.5
-
for formate oxidation Thiobacillus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thiobacillus sp.