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Literature summary for 1.17.1.9 extracted from

  • Niks, D.; Duvvuru, J.; Escalona, M.; Hille, R.
    Spectroscopic and kinetic properties of the molybdenum-containing, NAD+-dependent formate dehydrogenase from Ralstonia eutropha (2016), J. Biol. Chem., 291, 1162-1174.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cupriavidus necator 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
178800
-
 gel filtration Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Cupriavidus necator
-
 CO2 + NADH + H+
-
 ?
formate + NAD+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, butyl 4-Sepharose column chromatography, Cibacron Blue-agarose 3GA column chromatography, Fractogel TMAE 650 gel filtration, and Superdex 200 gel filtration Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Cupriavidus necator CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FdsABG
-
 Cupriavidus necator
NAD+-dependent formate dehydrogenase
-
 Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
molybdenum cofactor
-
 Cupriavidus necator
NAD+
-
 Cupriavidus necator