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Literature summary for 1.17.1.9 extracted from

  • Hartmann, T.; Leimkuehler, S.
    The oxygen-tolerant and NAD+-dependent formate dehydrogenase from Rhodobacter capsulatus is able to catalyze the reduction of CO2 to formate (2013), FEBS J., 280, 6083-6096.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not inactivated by molecular oxygen Rhodobacter capsulatus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.173
-
NAD+ at pH 9.0 and 30°C Rhodobacter capsulatus
0.277
-
formate with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
0.278
-
formate with ferricyanide as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
0.281
-
formate with NAD+ as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rhodobacter capsulatus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
-
Rhodobacter capsulatus
50000
-
-
Rhodobacter capsulatus
100000
-
-
Rhodobacter capsulatus
340000
-
gel filtration Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ Rhodobacter capsulatus
-
CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + 2,6-dichlorophenolindophenol
-
Rhodobacter capsulatus CO2 + reduced 2,6-dichlorophenolindophenol
-
?
formate + ferricyanide
-
Rhodobacter capsulatus CO2 + ferrocyanide
-
?
formate + NAD+
-
Rhodobacter capsulatus CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
heterotrimer 2 * 100000 + 2 * 50000 + 2 * 15000, SDS-PAGE Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
FDH
-
Rhodobacter capsulatus
NAD+-dependent formate dehydrogenase
-
Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
CO2 with NADH as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
32
-
formate with 2,6-dichlorophenolindophenol as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
36.5
-
formate with NAD+ as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus
39.7
-
formate with ferricyanide as cosubstrate, at pH 9.0 and 30°C Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
bis-molybdopterin guanine dinucleotide
-
Rhodobacter capsulatus
FMN
-
Rhodobacter capsulatus
NAD+
-
Rhodobacter capsulatus
[2Fe-2S]-center
-
Rhodobacter capsulatus
[4Fe-4S]-center
-
Rhodobacter capsulatus