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Literature summary for 1.17.1.9 extracted from

  • Liu, A.; Feng, R.; Liang, B.
    Microbial surface displaying formate dehydrogenase and its application in optical detection of formate (2016), Enzyme Microb. Technol., 91, 59-65.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Transetta (DE3) cells [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 61.51% residual activity at 10 mM [Candida] boidinii
CO32- 16.93% residual activity at 100 mM [Candida] boidinii
H2PO4- 78.54% residual activity at 100 mM [Candida] boidinii
Mg2+ 89.93% residual activity at 10 mM [Candida] boidinii
NO3- 40.89% residual activity at 100 mM [Candida] boidinii
Pb2+ 24.33% residual activity at 10 mM [Candida] boidinii
PO43- 30.9% residual activity at 100 mM [Candida] boidinii

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Li+, Na+, K+, NH4+, Cl?, HPO42-, HCO3-, and SO42- at 100 mM have scarcely effect on the enzymatic activity. The enzyme activity is very slightly increased in the presence of 1 mM Co2+, Ni2+, Cu2+, Zn2+, Fe3+, Mn2+, Mg2+, Ca2+, and NH4+ [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
-
 [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii O13437
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?
additional information no activity with methanol, formaldehyde, ethanol, sodium acetate, sodium propionate, sodium oxalate, and sodium citrate [Candida] boidinii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, enzyme fused to maltose binding protein and ice nucleation protein, SDS-PAGE [Candida] boidinii

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii
NAD+-dependent formate dehydrogenase
-
 [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 [Candida] boidinii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 more than 40% activity between 40 and 60°C [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 50 the Escherichia coli cell-surface-displayed enzyme retains its original enzymatic activity after incubation at 4°C for 1 month with the half-life of 17 days at 40°C and 38 h at 50°C [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 [Candida] boidinii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 more than 50% activity between pH 6.0 and 10.0 [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii