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Literature summary for 1.17.1.9 extracted from

  • Guo, Q.; Gakhar, L.; Wickersham, K.; Francis, K.; Vardi-Kilshtain, A.; Major, D.T.; Cheatum, C.M.; Kohen, A.
    Structural and kinetic studies of formate dehydrogenase from Candida boidinii (2016), Biochemistry, 55, 2760-2771.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells [Candida] boidinii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method [Candida] boidinii

Protein Variants

Protein Variants Comment Organism
K328V the mutant shows increased catalytic efficiency for NAD+ compared to the wild type enzyme [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
azide nanomolar inhibitor [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
0.0303
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
4
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
5
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii O13437
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose 6 column chromatography and Superdex 200 gel filtration [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FDH
-
 [Candida] boidinii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
5.6
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
6.8
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
6.8
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.4
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
180
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
270
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii