Protein Variants | Comment | Organism |
---|---|---|
C386S | inactive | Rhodobacter capsulatus |
H387F | inactive | Rhodobacter capsulatus |
H387K | the mutant shows about 6200fold decreased catalytic efficiency compared to the wild type enzyme | Rhodobacter capsulatus |
H387M | the mutant shows about 20fold decreased catalytic efficiency compared to the wild type enzyme | Rhodobacter capsulatus |
H387M/R587T | inactive | Rhodobacter capsulatus |
H387R | inactive | Rhodobacter capsulatus |
R587K | the mutant shows about 6200fold decreased catalytic efficiency compared to the wild type enzyme | Rhodobacter capsulatus |
R587T | inactive | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Rhodobacter capsulatus | |
nitrate | competitive inhibitor | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
formate | wild type enzyme, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
3.6 | - |
formate | mutant enzyme H387M, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
27.5 | - |
formate | mutant enzyme H387K, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
362 | - |
formate | mutant enzyme R587K, at pH 9.0 and 25°C | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Rhodobacter capsulatus | CO2 + NADH + H+ | - |
? | |
additional information | the enzyme also exhibits nitrate reductase activity with methyl viologen as cosubstrate | Rhodobacter capsulatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
formate | mutant enzyme H387K, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
11.2 | - |
formate | mutant enzyme R587K, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
33.7 | - |
formate | mutant enzyme H387M, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
35.4 | - |
formate | wild type enzyme, at pH 9.0 and 25°C | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdenum cofactor | - |
Rhodobacter capsulatus | |
NAD+ | - |
Rhodobacter capsulatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
nitrate | wild type enzyme, at pH 9.0 and 25°C | Rhodobacter capsulatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
formate | mutant enzyme R587K, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
0.033 | - |
formate | mutant enzyme H387K, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
9.38 | - |
formate | mutant enzyme H387M, at pH 9.0 and 25°C | Rhodobacter capsulatus | |
186.4 | - |
formate | wild type enzyme, at pH 9.0 and 25°C | Rhodobacter capsulatus |