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Literature summary for 1.17.1.9 extracted from
- Production and separation of formate dehydrogenase from Candida boidinii (2007), Enzyme Microb. Technol., 40, 940-946.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | adding 5 mM NAD+ in 0.2 M KCl improves the FDH elution and increases the specific FDH activity by 1.38fold as compared to elution with 1 M KCl | [Candida] boidinii |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] boidinii | - |
strain ATCC 32195 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation with polyethyleneimine at 0.04 mg/ml and Sepharose-Procion Blue HERB column chromatography | [Candida] boidinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
crude extract, at pH 7.4 and 25°C | [Candida] boidinii |
| 2.63 | - |
after 5.5fold purification, at pH 7.4 and 25°C | [Candida] boidinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FDH | - |
[Candida] boidinii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
[Candida] boidinii | |
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