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Literature summary for 1.17.1.9 extracted from
- Characterization of recombinant Aspergillus fumigatus mannitol-1-phosphate 5-dehydrogenase and its application for the stereoselective synthesis of protio and deuterio forms of D-mannitol 1-phosphate (2008), Carbohydr. Res., 343, 1414-1423.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells | [Candida] boidinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] boidinii | O13437 | strain ATCC 18810 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification, Q-Sepharose column chromatography | [Candida] boidinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
crude extract, in 30 mM Tris-HCl, pH 7.5. at 25°C | [Candida] boidinii |
| 4.4 | - |
recombinant enzyme, after 4.5fold purification, in 30 mM Tris-HCl, pH 7.5. at 25°C | [Candida] boidinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formate + NAD+ | - |
[Candida] boidinii | CO2 + NADH + H+ | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FDH | - |
[Candida] boidinii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
[Candida] boidinii | |
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Release 2023.1 (January 2023)
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