Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethyl acetate | wild-type, 21% increase of activity | Mycolicibacterium vaccae |
Application | Comment | Organism |
---|---|---|
biotechnology | use of enzyme for regeneration of NADH. Coexpression of alpha-haloketone resistant enzyme mutants and a carbonyl reductase from Kluyveromyces aestuarii for production of ethyl-(S)-4-chloro-3-hydroxybutanoate with optical purity of product of 99% enantiomeric excess, from ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
Protein Variants | Comment | Organism |
---|---|---|
C146S/C256V | 85% increase of activity in presence of ethyl acetate, 104% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C256V | 11% increase of activity in presence of ethyl acetate, 100% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6A/C146A/C256V | 119% increase of activity in presence of ethyl acetate, 106% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6A/C146S/C256V | 37% increase of activity in presence of ethyl acetate, 108% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6A/C146V/C256V | 15% decrease of activity in presence of ethyl acetate, 100% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6A/C256S | 9% increase of activity in presence of ethyl acetate, 97% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6A/C256V | 11% increase of activity in presence of ethyl acetate, 94.5% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6S/C256A | 25% increase of activity in presence of ethyl acetate, 120% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6S/C256S | 5% increase of activity in presence of ethyl acetate, 125% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6S/C256V | 10% increase of activity in presence of ethyl acetate, 94% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
C6V/C256S | 5% increase of activity in presence of ethyl acetate, 92% residual activity in presence of 20 mM ethyl-4-chloroacetoacetate | Mycolicibacterium vaccae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethyl-4-chloroacetoacetate | wild-type, 7.43% residual activity at 20 mM | Mycolicibacterium vaccae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium vaccae | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.285 | - |
mutant C6S/C256S, pH 6.5, 30°C | Mycolicibacterium vaccae |
0.504 | - |
mutant C6S/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
0.635 | - |
mutant C6S/C256A, pH 6.5, 30°C | Mycolicibacterium vaccae |
1.15 | - |
mutant C6A/C146A/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
1.49 | - |
mutant C6A/C146V/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
1.62 | - |
mutant C6V/C256S, pH 6.5, 30°C | Mycolicibacterium vaccae |
1.83 | - |
mutant C6A/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
1.98 | - |
mutant C6A/C256S, pH 6.5, 30°C | Mycolicibacterium vaccae |
2.29 | - |
mutant C146S/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
2.58 | - |
mutant C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
2.65 | - |
mutant C6A/C146S/C256V, pH 6.5, 30°C | Mycolicibacterium vaccae |
3.24 | - |
wild-type, pH 6.5, 30°C | Mycolicibacterium vaccae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Mycolicibacterium vaccae | CO2 + NADH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycolicibacterium vaccae | |
NADH | - |
Mycolicibacterium vaccae |