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Literature summary for 1.17.1.9 extracted from

  • Shinoda, T.; Arai, K.; Shigematsu-Iida, M.; Ishikura, Y.; Tanaka, S.; Yamada, T.; Kimber, M.S.; Pai, E.F.; Fushinobu, S.; Taguchi, H.
    Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase (2005), J. Biol. Chem., 280, 17068-17075.
    View publication on PubMed
Search for more literature for 1.17.1.9

Protein Variants

Protein Variants Comment Organism
E141N 4.3fold decrease in Km value, 590fold decrease in kcat value for formate, marked enhancement of glyoxylate reducing activity, conversion of enzyme to a 2-hydroxy acid dehydrogenase Paracoccus sp.
E141Q 5.5fold decrease in Km value, 110fold decrease in kcat value for formate Paracoccus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
 glyoxylate mutant E141Q, 30°C, pH 7.0 Paracoccus sp.
4.6
-
 glyoxylate mutant E141N, 30°C, pH 7.0 Paracoccus sp.
5.6
-
 formate wild-type, 30°C, pH 7.0 Paracoccus sp.
7.5
-
 glyoxylate wild-type, 30°C, pH 7.0 Paracoccus sp.
25
-
 formate mutant E141N, 30°C, pH 7.0 Paracoccus sp.
31
-
 formate mutant E141Q, 30°C, pH 7.0 Paracoccus sp.

Organism

Organism UniProt Comment Textmining
Paracoccus sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
formate + NAD+ = CO2 + NADH + H+ hydride-transfer step is rate-limiting Paracoccus sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Paracoccus sp. CO2 + NADH + H+
-
 ?
glyoxylate + NAD+ wild-type, low activity towards glyoxylate Paracoccus sp. ? + NADH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 glyoxylate wild-type, 30°C, pH 7.0 Paracoccus sp.
0.01
-
 formate mutant E141N, 30°C, pH 7.0 Paracoccus sp.
0.023
-
 glyoxylate mutant E141Q, 30°C, pH 7.0 Paracoccus sp.
0.054
-
 formate mutant E141Q, 30°C, pH 7.0 Paracoccus sp.
0.36
-
 glyoxylate mutant E141N, 30°C, pH 7.0 Paracoccus sp.
6.1
-
 formate wild-type, 30°C, pH 7.0 Paracoccus sp.