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Literature summary for 1.17.1.9 extracted from

  • Nanba, H.; Takaoka, Y.; Hasegawa, J.
    Purification and characterization of an alpha-haloketone-resistant formate dehydrogenase from Thiobacillus sp. strain KNK65MA, and cloning of the gene (2003), Biosci. Biotechnol. Biochem., 67, 2145-2153.
    View publication on PubMed
Search for more literature for 1.17.1.9

Cloned(Commentary)

Cloned (Comment) Organism
high expression in Escherichia coli Thiobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-2-oxopropane 20 mM, pH 6.5, 30°C, 5 h, 76% loss of activity Thiobacillus sp.
2-chloro-1-(3-pyridyl)-ethanone 20 mM, pH 6.5, 30°C, 5 h, 10% loss of activity Thiobacillus sp.
Cu2+ 0.2 mM, 13.6% inhibition Thiobacillus sp.
Hg2+ 1 mM, complete inhibition Thiobacillus sp.
additional information no loss of activity after 5 h at pH 6.5, 30°C, after 5 h with 20 mM ethyl 4-chloro-3-oxobutanoate, 20 mM ethyl-4-bromo-3-oxobutanoate, 20 mM ethyl 2-chloro-3-oxobutanoate, or 20 mM 2,3'-dichloroacetophenone Thiobacillus sp.
NaN3 1 mM, complete inhibition Thiobacillus sp.
PCMB 0.2 mM, 10.7% inhibition Thiobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 NAD+ pH 7.0, 30°C Thiobacillus sp.
1.6
-
 formate pH 7.0, 30°C Thiobacillus sp.

Organism

Organism UniProt Comment Textmining
Thiobacillus sp.
-
-
-
Thiobacillus sp. KNK65MA
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thiobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.53
-
-
 Thiobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+
-
 Thiobacillus sp. CO2 + NADH + H+
-
 ?
formate + NAD+
-
 Thiobacillus sp. KNK65MA CO2 + NADH + H+
-
 ?
formate + NADP+ 4% of the activity with NAD+ Thiobacillus sp. CO2 + NADPH
-
 ?
formate + NADP+ 4% of the activity with NAD+ Thiobacillus sp. KNK65MA CO2 + NADPH
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Thiobacillus sp.

Synonyms

Synonyms Comment Organism
FDH
-
 Thiobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
-
 Thiobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 65 40°C: about 40% of maximal activity, 65°C: about 30% of maximal activity Thiobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, 10 min, stable at or below Thiobacillus sp.
55
-
 pH 7.0, 10 min, about 55% loss of activity Thiobacillus sp.
60
-
 pH 7.0, 10 min, complete loss of activity Thiobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Thiobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 80% of maximal activity, pH 10.0: about 65% of maximal activity Thiobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 30°C, 22h, about 75% loss of activity Thiobacillus sp.
5
-
 30°C, 22h, about 20% loss of activity Thiobacillus sp.
6 8 30°C, 22 h, stable Thiobacillus sp.
8.5
-
 30°C, 22h, about 25% loss of activity Thiobacillus sp.
9
-
 30°C, 22h, about 55% loss of activity Thiobacillus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thiobacillus sp.
NADP+ 4.2% of the activity with NAD+ Thiobacillus sp.