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Literature summary for 1.17.1.9 extracted from

  • Serov, A.E.; Popova, A.S.; Fedorchuk, V.V.; Tishkov, V.I.
    Engineering of coenzyme specificity of formate dehydrogenase from Saccharomyces cerevisiae (2002), Biochem. J., 367, 841-847.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

Protein Variants

Protein Variants Comment Organism
D196A/Y197R coenzyme preference is shifted from NAD+ to NADP+, decreased thermal stability at 42°C Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0365
-
 NAD+ pH 7.0, 30°C, wild-type enzyme Saccharomyces cerevisiae
0.13
-
 NADP+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate Saccharomyces cerevisiae
0.16
-
 NADP+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate Saccharomyces cerevisiae
5.5
-
 formate pH 7.0, 30°C, wild-type enzyme Saccharomyces cerevisiae
7.6
-
 NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.25 M formate Saccharomyces cerevisiae
8.4
-
 NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate Saccharomyces cerevisiae
1000
-
 formate pH 7.0, 30°C, mutant enzyme D196A/Y197R, reaction with NADP+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08911
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + NAD+ wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ Saccharomyces cerevisiae CO2 + NADH + H+
-
 ?
formate + NADP+ wild-type enzyme shows no activity, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ Saccharomyces cerevisiae CO2 + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
SceFDH
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 wild-type enzyme loses 50% of its activity after 3.5 min, mutant enzyme D196A/Y197R loses 50% of its activity after 1.5 min Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.095
-
 NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R Saccharomyces cerevisiae
0.12
-
 NAD+ pH 7.0, 30°C, mutant enzyme D196A/Y197R, with 0.5 M formate Saccharomyces cerevisiae
4.5
-
 NADP+ pH 7.0, 30°C, mutant enzyme D196A/Y197R Saccharomyces cerevisiae
6.5
-
 NAD+ pH 7.0, 30°C, wild-type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NAD+ wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ Saccharomyces cerevisiae
NADP+ wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ Saccharomyces cerevisiae