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Literature summary for 1.17.1.9 extracted from

  • Friedebold, J.; Bowien, B.
    Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus (1993), J. Bacteriol., 175, 4719-4728.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.1.9

General Stability

General Stability Organism
N3- and NO3- stabilize Cupriavidus necator

Inhibitors

Inhibitors Comment Organism Structure
CN-
-
 Cupriavidus necator
Hg2+
-
 Cupriavidus necator
N3-
-
 Cupriavidus necator
NO3- 10 mM KNO3, 38% inhibition Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 NAD+
-
 Cupriavidus necator
3.3
-
 formate
-
 Cupriavidus necator

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum 0.64 gatom per mol of enzyme. The enzyme contains molybdopterin guanine dinucleotide Cupriavidus necator

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11600
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
19400
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
57000
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
110000
-
 alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator
197000
-
 non-denaturing gradient PAGE Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cupriavidus necator

Storage Stability

Storage Stability Organism
-20°C, stable for several weeks Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formate + 2,6-dichloroindophenol 54% of the activity with NAD+ Cupriavidus necator CO2 + reduced 2,6-dichlorophenolindophenol
-
 ?
formate + benzyl viologen 113% of the activity with NAD+ Cupriavidus necator CO2 + reduced benzyl viologen
-
 ?
formate + ferricyanide 10% of the activity with NAD+ Cupriavidus necator CO2 + ferrocyanide
-
 ?
formate + methyl viologen 74% of the activity with NAD+ Cupriavidus necator CO2 + reduced methyl viologen
-
 ?
formate + methylene blue 119% of the activity with NAD+ Cupriavidus necator CO2 + reduced methylene blue
-
 ?
formate + NAD+
-
 Cupriavidus necator CO2 + NADH + H+
-
 ?
formate + nitrobluetetrazolium 54% of the activity with NAD+ Cupriavidus necator CO2 + reduced nitrobluetetrazolium
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha,beta,gamma,delta, 1 * 110000 + 1 * 57000 + 1 * 19400 + 1 * 11600, SDS-PAGE Cupriavidus necator

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 rapid inactivation above Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 phosphate buffer Cupriavidus necator
7.9
-
 Tris-HCl buffer Cupriavidus necator

pH Range

pH Minimum pH Maximum Comment Organism
5 10 completely inactive below pH 5.5 and above pH 10.0 Cupriavidus necator

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 optimal stability in presence of 10 mM KNO3 Cupriavidus necator
8
-
 rapid decrease of stability above Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
FMN 0.9 mol of FMN per mol of enzyme Cupriavidus necator
NAD+
-
 Cupriavidus necator