Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
[Candida] methylica | |
Dithionitrobenzoate | - |
[Candida] methylica | |
N3- | - |
[Candida] methylica | |
PCMB | - |
[Candida] methylica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NAD+ | - |
[Candida] methylica | |
13 | - |
formate | - |
[Candida] methylica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000, SDS-PAGE | [Candida] methylica |
70000 | - |
gel filtration | [Candida] methylica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | [Candida] methylica | induced by methanol and formate | CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] methylica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two-step procedure and single-step procedure | [Candida] methylica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
- |
[Candida] methylica |
Storage Stability | Organism |
---|---|
4°C, enzyme dissolved in glycerol, retains activity for a few months | [Candida] methylica |
4°C, pH 7.6, in presence of cofactor, EDTA, 2-mercaptoethanol and glycerol, 50% loss of activity after 72 h. NADH decrease the inactivation constant 2.5fold, formate decreases the inactivation constant 5fold, 2-mercaptoethanol decreases the inactivation constant 8fold | [Candida] methylica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
[Candida] methylica | CO2 + NADH + H+ | - |
? | |
formate + NAD+ | induced by methanol and formate | [Candida] methylica | CO2 + NADH + H+ | - |
? | |
formate + phenazine methosulfate | no activity | [Candida] methylica | CO2 + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000, SDS-PAGE | [Candida] methylica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Candida] methylica |