Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | activates less than phosphate | Clostridium sp. | |
methylphosphate | activates less than phosphate | Clostridium sp. | |
additional information | not activated by high concentrations of citrate, sulfate, or diphosphate | Clostridium sp. | |
phosphate | slight activation, half-maximal activation at 50 mM | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADP+ | - |
Clostridium sp. | |
0.11 | - |
nicotinate | - |
Clostridium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Clostridium sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
analytical ultracentrifugation | Clostridium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + NADP+ | Clostridium sp. | first step in the anaerobic decomposition of nicotinate, NADP+ may be the natural, primary electron acceptor | 6-hydroxynicotinate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sp. | - |
nicotinic acid-fermenting | - |
Purification (Comment) | Organism |
---|---|
24fold increase in specific activity | Clostridium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.8 | - |
- |
Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pyrazinecarboxylate + H2O + NADP+ | 30% of the activity with nicotinate | Clostridium sp. | ? | - |
r | |
additional information | not as substrates: nicotinamide, isonicotinate, picolinate, nicotinic acid N-oxide, N-methylnicotinamide, trigonelline, hypoxanthine, not as electron acceptor: NAD+ | Clostridium sp. | ? | - |
? | |
nicotinate + H2O + NADP+ | specific for NADP+, but artificial electron acceptors, i.e. benzyl viologen and 2,3,5-triphenyltetrazolium dyes, can replace NADP+, high substrate specificity with respect to nicotinate, requiring an unsubstituted pyridine or pyrazine ring nitrogen and a carboxyl group meta to the nitrogen | Clostridium sp. | 6-hydroxynicotinate + NADPH | - |
r | |
nicotinate + H2O + NADP+ | equilibrium lies far in direction of 6-hydroxynicotinate formation | Clostridium sp. | 6-hydroxynicotinate + NADPH | - |
r | |
nicotinate + H2O + NADP+ | first step in the anaerobic decomposition of nicotinate, NADP+ may be the natural, primary electron acceptor | Clostridium sp. | 6-hydroxynicotinate + NADPH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hydroxylase activity is more sensitive to heat inactivation than are diaphorase and NADPH-oxidase activities | Clostridium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | phosphate buffer | Clostridium sp. |
8 | - |
triethanolamine phosphate buffer | Clostridium sp. |
8 | 8.5 | triethanolamine buffer | Clostridium sp. |
8.3 | 9 | Tris buffer or diphosphate buffer | Clostridium sp. |
8.5 | - |
dimethylglutarate-phosphate buffer | Clostridium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
benzyl viologen | can replace NADP+ | Clostridium sp. | |
FAD | FAD as prosthetic group, 1.5 mol flavin per mol of enzyme | Clostridium sp. | |
additional information | NAD+/NADH can not replace NADP+/NADPH | Clostridium sp. | |
additional information | enzyme contains 11 mol non-heme iron and 6 mol acid-labile sulfide per mole of enzyme | Clostridium sp. | |
NADP+ | specific for NADP+, artificial electron acceptors, i.e. benzyl viologen and 2,3,5-triphenyltetrazolium dyes, can replace NADP+ | Clostridium sp. | |
NADPH | NADPH oxidase activity | Clostridium sp. |