Any feedback?
Literature summary for 1.17.1.4 extracted from
- Human Xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate (2007), J. Biochem., 141, 513-524.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant E803V, decrease in activity towards purine substrates is not due to large conformational change in the mutant protein | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E803V | almost complete loss of activity with hypoxanthine, weak activity with xanthine, significant aldehyde oxidase activity | Homo sapiens |
| R881M | almost complete loss of activity with xanthine, weak activity with hypoxanthine, significant aldehyde oxidase activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0088 | - |
hypoxanthine | wild-type, pH 8.5, 25°C | Homo sapiens |  |
| 0.0088 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
| 0.072 | - |
xanthine | mutant E803V, pH 8.5, 25°C | Homo sapiens | |
| 0.072 | - |
hypoxanthine | mutant R881M, pH 8.5, 25°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| after expression in Escherichia coli | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + NAD+ + H+ + O2- + H2O | - |
Homo sapiens | xanthine + NADH + H2O2 | - |
? | |
| xanthine + NAD+ + H2O | - |
Homo sapiens | urate + NADH + H+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
hypoxanthine | mutant R881M, pH 8.5, 25°C | Homo sapiens | |
| 0.99 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
| 1.35 | - |
xanthine | mutant E803V, pH 8.5, 25°C | Homo sapiens | |
| 18.33 | - |
xanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
| 30 | - |
hypoxanthine | wild-type, pH 8.5, 25°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
