Application | Comment | Organism |
---|---|---|
synthesis | for maximal level of functional expression, co-expression of xdhC gene is required which increases level of molybdenum incorporation. Iron and FAD content of expressed enzymes are independent of xdhC expression | Delftia acidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 3.7-3.9 atoms per alphabeta protomer | Delftia acidovorans | |
Molybdenum | 1.1 atoms per alphabeta protomer in wild-type, 0.89 atoms per alphabeta protomer after expression of xdhABC genes, 0.24 atoms per alphabeta protomer after expression of xdhAB genes | Delftia acidovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
expression in Pseudomonas aeruginosa, for maximal level of functional expression, co-expression of xdhC gene is required which increases level of molybdenum incorporation. Iron and FAD content of expressed enzymes are independent of xdhC expression | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.58 | - |
pH 7.8, 25°C, expression of xdhAB genes | Delftia acidovorans |
80 | - |
pH 7.8, 25°C, expression of xdhABC genes | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + NADH | - |
Delftia acidovorans | xanthine + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1.0-1.1 molecules per alphabeta protomer | Delftia acidovorans |