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Literature summary for 1.17.1.4 extracted from

  • Leimkuhler, S.; Hodson, R.; George, G.N.; Rajagopalan, K.V.
    Recombinant Rhodobacter capsulatus xanthine dehydrogenase, a useful model system for the characterization of protein variants leading to xanthinuria I in humans (2003), J. Biol. Chem., 278, 20802-20811.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
R135C mutation corresponding to human protein variant of a patient suffering from xanthinuria I. Mutation results in an active (alphabeta)2 heterotetrameric form besides an inactive alphabeta heterodimeric form missing the FeSI center Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron FeS center, EPR spectra, wild type 3.7 mol per subunit, active form of mutant R135C 2.8 mol per subunit, inactive form of mutant R135C 1.2 mol per subunit Rhodobacter capsulatus
Molybdenum active form of mutant R135C Moco content 97%, inactive form of mutant R135C Moco content 3.8% Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rhodobacter capsulatus
-
expression in Escherichia coli
-

Source Tissue

Source Tissue Comment Organism Textmining
milk
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2,6-dichloroindophenol + H2O 0.1% of activity with xanthine Rhodobacter capsulatus ?
-
?
acetaldehyde + 2,6-dichloroindophenol + H2O 1.2% of activity with xanthine Bos taurus ?
-
?
glyceraldehyde + 2,6-dichloroindophenol + H2O 0.3% of activity with xanthine Rhodobacter capsulatus ?
-
?
glyceraldehyde + 2,6-dichloroindophenol + H2O 12.1% of activity with xanthine Bos taurus ?
-
?
hypoxanthine + NAD+ + H+ + O2- + H2O 12.4% of activity with xanthine Bos taurus xanthine + NADH + H2O2
-
?
hypoxanthine + NAD+ + H+ + O2- + H2O 19% of activity with xanthine Rhodobacter capsulatus xanthine + NADH + H2O2
-
?
pterin + 2,6-dichloroindophenol + H2O 22.7% of activity with xanthine Bos taurus ?
-
?
pterin + 2,6-dichloroindophenol + H2O 9.7% of activity with xanthine Rhodobacter capsulatus ?
-
?
purine + 2,6-dichloroindophenol + H2O 18.7% of activity with xanthine Bos taurus ?
-
?
purine + 2,6-dichloroindophenol + H2O 8.5% of activity with xanthine Rhodobacter capsulatus ?
-
?
xanthine + NAD+ + H2O
-
Bos taurus urate + NADH
-
?
xanthine + NAD+ + H2O
-
Rhodobacter capsulatus urate + NADH
-
?

Cofactor

Cofactor Comment Organism Structure
FAD 0.9 mol per subunit Rhodobacter capsulatus