Protein Variants | Comment | Organism |
---|---|---|
R135C | mutation corresponding to human protein variant of a patient suffering from xanthinuria I. Mutation results in an active (alphabeta)2 heterotetrameric form besides an inactive alphabeta heterodimeric form missing the FeSI center | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | FeS center, EPR spectra, wild type 3.7 mol per subunit, active form of mutant R135C 2.8 mol per subunit, inactive form of mutant R135C 1.2 mol per subunit | Rhodobacter capsulatus | |
Molybdenum | active form of mutant R135C Moco content 97%, inactive form of mutant R135C Moco content 3.8% | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rhodobacter capsulatus | - |
expression in Escherichia coli | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + 2,6-dichloroindophenol + H2O | 0.1% of activity with xanthine | Rhodobacter capsulatus | ? | - |
? | |
acetaldehyde + 2,6-dichloroindophenol + H2O | 1.2% of activity with xanthine | Bos taurus | ? | - |
? | |
glyceraldehyde + 2,6-dichloroindophenol + H2O | 0.3% of activity with xanthine | Rhodobacter capsulatus | ? | - |
? | |
glyceraldehyde + 2,6-dichloroindophenol + H2O | 12.1% of activity with xanthine | Bos taurus | ? | - |
? | |
hypoxanthine + NAD+ + H+ + O2- + H2O | 12.4% of activity with xanthine | Bos taurus | xanthine + NADH + H2O2 | - |
? | |
hypoxanthine + NAD+ + H+ + O2- + H2O | 19% of activity with xanthine | Rhodobacter capsulatus | xanthine + NADH + H2O2 | - |
? | |
pterin + 2,6-dichloroindophenol + H2O | 22.7% of activity with xanthine | Bos taurus | ? | - |
? | |
pterin + 2,6-dichloroindophenol + H2O | 9.7% of activity with xanthine | Rhodobacter capsulatus | ? | - |
? | |
purine + 2,6-dichloroindophenol + H2O | 18.7% of activity with xanthine | Bos taurus | ? | - |
? | |
purine + 2,6-dichloroindophenol + H2O | 8.5% of activity with xanthine | Rhodobacter capsulatus | ? | - |
? | |
xanthine + NAD+ + H2O | - |
Bos taurus | urate + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Rhodobacter capsulatus | urate + NADH | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.9 mol per subunit | Rhodobacter capsulatus |