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Literature summary for 1.17.1.4 extracted from

  • Smith, S.T.; Rajagopalan, K.V.; Handler, P.
    Purification and properties of xanthine dehydrogenase from Micrococcus lactilyticus (1967), J. Biol. Chem., 242, 4108-4117.
    View publication on PubMed
Search for more literature for 1.17.1.4

Activating Compound

Activating Compound Comment Organism Structure
Ferredoxin 26fold increase of cytochrome c reduction, 2fold increase of oxygen reduction Veillonella atypica

Inhibitors

Inhibitors Comment Organism Structure
8-Azaxanthine 50% inhibition of ferricyanide reduction in xanthine oxidation assay at 5 mM Veillonella atypica
KCN 10-50% inhibition of xanthine oxidation only in the presence of Tris or phosphate buffers from 0.01 to 0.1 M inhibitor concentration Veillonella atypica
methanol develops inhibition during course of catalysis, enhanced inhibition in the presence of ferricyanide in the oxygen-dependent oxidation of xanthine Veillonella atypica
Urate 50% inhibition of xanthine oxidation at 0.5 mM Veillonella atypica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
 Veillonella atypica
Mo
-
 Veillonella atypica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
 sucrose density gradient centrifugation Veillonella atypica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,8-dihydroxypurine + O2 Veillonella atypica
-
 ? + H2O2
-
 ?
hypoxanthine + O2 Veillonella atypica
-
 xanthine + H2O2
-
 ?
hypoxanthine + urate Veillonella atypica oxygen-free assay xanthine + 6,8-dihydroxypurine
-
 r
purine + NAD+ + H2O Veillonella atypica
-
 8-hydroxypurine + NADH + H+
-
 ?
xanthine + O2 Veillonella atypica dismutation reaction hypoxanthine + ?
-
 ?
xanthine + O2 + H2O Veillonella atypica presence of ferredoxin enhances rate of oxygen reduction urate + O2- + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Veillonella atypica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, precipitation and chromatography techniques Veillonella atypica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Veillonella atypica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
-
 Veillonella atypica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dichloroindophenol + NADH + H+
-
 Veillonella atypica reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
6,8-dihydroxypurine + O2
-
 Veillonella atypica ? + H2O2
-
 ?
6,8-dihydroxypurine + O2 + H2O
-
 Veillonella atypica ? + H2O2
-
 ?
9-methylhypoxanthine + O2
-
 Veillonella atypica 9-methylxanthine + H2O2
-
 ?
benzaldehyde + O2
-
 Veillonella atypica benzoate + H2O2
-
 ?
hypoxanthine + O2
-
 Veillonella atypica xanthine + H2O2
-
 ?
hypoxanthine + urate oxygen-free assay Veillonella atypica xanthine + 6,8-dihydroxypurine
-
 r
purine + NAD+ + H2O
-
 Veillonella atypica 8-hydroxypurine + NADH + H+
-
 ?
salicylaldehyde + O2
-
 Veillonella atypica salicylate + H2O2
-
 r
xanthine + cytochrome c + H2O presence of ferredoxin enhances cytochrom c reduction Veillonella atypica urate + reduced cytochrome c
-
 ?
xanthine + ferredoxin + H2O
-
 Veillonella atypica urate + reduced ferredoxin
-
 ?
xanthine + ferricyanide + H2O
-
 Veillonella atypica urate + ferrocyanide
-
 ?
xanthine + methylene blue + H2O
-
 Veillonella atypica urate + reduced methylene blue
-
 ?
xanthine + myoglobin + H2O
-
 Veillonella atypica urate + reduced myoglobin
-
 ?
xanthine + nitroblue tetrazolium + H2O
-
 Veillonella atypica urate + ?
-
 ?
xanthine + O2 dismutation reaction Veillonella atypica hypoxanthine + ?
-
 ?
xanthine + O2 + H2O presence of ferredoxin enhances rate of oxygen reduction Veillonella atypica urate + O2- + 2 H+
-
 ?
xanthine + phenazine methosulfate + H2O
-
 Veillonella atypica urate + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Salicylaldehyde electron acceptor: oxygen Veillonella atypica
0.7
-
 purine electron acceptor: oxygen Veillonella atypica
0.717
-
 cytochrome c cosubstrate xanthine Veillonella atypica
0.75
-
 hypoxanthine electron acceptor: oxygen Veillonella atypica
0.8
-
 oxygen cosubstrate xanthine Veillonella atypica
2.5
-
 xanthine electron acceptor: oxygen Veillonella atypica
5.67
-
 2,6-dichlorophenolindophenol cosubstrate xanthine Veillonella atypica
9.55
-
 Nitro blue tetrazolium cosubstrate xanthine Veillonella atypica
18.2
-
 ferricyanide cosubstrate xanthine Veillonella atypica
20.2
-
 Ferredoxin cosubstrate xanthine Veillonella atypica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 xanthine oxidation, presence of oxygen, two pH-optima Veillonella atypica
9.5
-
 xanthine oxidation, presence of oxygen, two pH-optima Veillonella atypica

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 unstable below, stabilized and activated at higher pH values Veillonella atypica

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Veillonella atypica
additional information molybdoironflavoprotein: 8 : 8 : 2 : 1.5 ratio of iron to sulfide to flavin to molybdenum Veillonella atypica