BRENDA - Enzyme Database show
show all sequences of 1.17.1.3

Isolation and characterization of genes encoding leucoanthocyanidin reductase (FeLAR) and anthocyanidin reductase (FeANR) in buckwheat (Fagopyrum esculentum)

Matsui, K.; Hisano, T.; Yasui, Y.; Mori, M.; Walker, A.R.; Morishita, T.; Katsu, K.; J. Plant Physiol. 205, 41-47 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene LAR1, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis; gene LAR2, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis; gene LAR3, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis
Fagopyrum esculentum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Fagopyrum esculentum
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Fagopyrum esculentum
A0A1B4ZAW1
cvs. Sachiizumi, Harunoibuki, 2SL05-1, Asahimurazairai, and Asahimurazairai/Kyushu PL4, from Japan
-
Fagopyrum esculentum
A0A1B4ZAW2
cvs. Sachiizumi, Harunoibuki, 2SL05-1, Asahimurazairai, and Asahimurazairai/Kyushu PL4, from Japan
-
Fagopyrum esculentum
A0A1B4ZAW7
cvs. Sachiizumi, Harunoibuki, 2SL05-1, Asahimurazairai, and Asahimurazairai/Kyushu PL4, from Japan
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bud
-
Fagopyrum esculentum
-
cotyledon
-
Fagopyrum esculentum
-
flower
-
Fagopyrum esculentum
-
leaf
-
Fagopyrum esculentum
-
additional information
tissue-specific expression of isozymes LAR1-LAR3, FeLAR1 is expressed mainly in stems, leaves, buds, flowers, and 2nd- and 3rd-stage seeds; tissue-specific expression of isozymes LAR1-LAR3, FeLAR2 is expressed very strongly inbuds; tissue-specific expression of isozymes LAR1-LAR3, FeLAR3 is expressed mainly in 1st- and 2nd-stage seeds
Fagopyrum esculentum
-
root
-
Fagopyrum esculentum
-
seed
-
Fagopyrum esculentum
-
seedling
-
Fagopyrum esculentum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
745634
Fagopyrum esculentum
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Fagopyrum esculentum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene LAR1, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis
Fagopyrum esculentum
gene LAR2, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis
Fagopyrum esculentum
gene LAR3, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis
Fagopyrum esculentum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Fagopyrum esculentum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
Fagopyrum esculentum
-
(2R,3S)-catechin + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bud
-
Fagopyrum esculentum
-
cotyledon
-
Fagopyrum esculentum
-
flower
-
Fagopyrum esculentum
-
leaf
-
Fagopyrum esculentum
-
additional information
tissue-specific expression of isozymes LAR1-LAR3, FeLAR1 is expressed mainly in stems, leaves, buds, flowers, and 2nd- and 3rd-stage seeds
Fagopyrum esculentum
-
additional information
tissue-specific expression of isozymes LAR1-LAR3, FeLAR2 is expressed very strongly inbuds
Fagopyrum esculentum
-
additional information
tissue-specific expression of isozymes LAR1-LAR3, FeLAR3 is expressed mainly in 1st- and 2nd-stage seeds
Fagopyrum esculentum
-
root
-
Fagopyrum esculentum
-
seed
-
Fagopyrum esculentum
-
seedling
-
Fagopyrum esculentum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
745634
Fagopyrum esculentum
(2R,3S)-catechin + NADP+ + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the proanthocyanidin biosynthesis by forming (+)-catechin, which polymerizes to proanthocyanidins, overview; the enzyme is involved in the proanthocyanidin biosynthesis by forming (+)-catechin, which polymerizes to proanthocyanidins, overview; the enzyme is involved in the proanthocyanidin biosynthesis by forming (+)-catechin, which polymerizes to proanthocyanidins, overview
Fagopyrum esculentum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the proanthocyanidin biosynthesis by forming (+)-catechin, which polymerizes to proanthocyanidins, overview
Fagopyrum esculentum
Other publictions for EC 1.17.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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700789
Gagne
Leucoanthocyanidin reductase a ...
Vitis vinifera
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2009
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2
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6
1
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6
1
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1
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676629
Paolocci
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1
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Pfeiffer
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Malus domestica, Vitis vinifera
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2006
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15
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3
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2
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