Any feedback?
Literature summary for 1.17.1.3 extracted from
- Synthesis of 3,4-cis[3H]leucocyanidin and enzymic reduction to catechin (1993), Anal. Biochem., 209, 274-277.
General Stability
| General Stability | Organism |
|---|---|
| only little loss of activity after freezing and thawing | Onobrychis viciifolia |
| unstable in desalted extracts | Hordeum vulgare |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
NADPH | pH 6.8, 30°C | Onobrychis viciifolia |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-trans-3,4-cis-leucocyanidin + NADPH | Hordeum vulgare | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? | |
| 2,3-trans-3,4-cis-leucocyanidin + NADPH | Onobrychis viciifolia | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
| Onobrychis viciifolia | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| caryopsis | - |
Hordeum vulgare | - |
| leaf | - |
Onobrychis viciifolia | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, desalted extract, 40% loss of activity after 90 min | Hordeum vulgare |
| 4°C, desalted extract, only little loss of activity after 90 min | Onobrychis viciifolia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-trans-3,4-cis-leucocyanidin + NADPH | - |
Hordeum vulgare | (2R,3S)-catechin + NADP+ + H2O | - |
? | |
| 2,3-trans-3,4-cis-leucocyanidin + NADPH | - |
Onobrychis viciifolia | (2R,3S)-catechin + NADP+ + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
- |
Hordeum vulgare |
| 6.6 | - |
- |
Onobrychis viciifolia |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | 80% activity at pH 6 and pH 7.5 | Hordeum vulgare |
| 6 | 7.5 | 80% activity at pH 6 and pH 7.5 | Onobrychis viciifolia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Hordeum vulgare | |
| NADH | - |
Onobrychis viciifolia | |
| NADPH | preferred over NADH | Hordeum vulgare | |
| NADPH | preferred over NADH | Onobrychis viciifolia | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
