Cloned (Comment) | Organism |
---|---|
- |
Desmodium uncinatum |
determination of DNA and amino acid sequences, expression in Escherichia coli strain XL-1 Blue, Nicotiana tabacum, and Trifolium repens, plant transformations via Agrobacterium tumefaciens infection system | Desmodium uncinatum |
Crystallization (Comment) | Organism |
---|---|
near homogeneity | Desmodium uncinatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-cis-flavan-3,4-diol | slight inhibition | Desmodium uncinatum | |
2,3-cis-flavan-3-ol | product inhibition | Desmodium uncinatum | |
2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxychromenium | 48% inhibition at 0.006 mM | Desmodium uncinatum | |
3,4-trans-leucocyanidin | 50% inhibition at 0.12 mM | Desmodium uncinatum | |
3,4-trans-leucopelargonidin | 50% inhibition at 0.46 mM | Desmodium uncinatum | |
afzelechin | 50% inhibition at 0.014 mM | Desmodium uncinatum | |
catechin | 50% inhibition at 0.012 mM | Desmodium uncinatum | |
delphinidin | 97% inhibition at 0.06 mM | Desmodium uncinatum | |
dihydroquercetin | 60% inhibition at 0.01 mM | Desmodium uncinatum | |
epi-gallocatechin | 50% inhibition at 1.4 mM | Desmodium uncinatum | |
eriodictyol | 53% inhibition at 0.01 mM | Desmodium uncinatum | |
gallocatechin | 50% inhibition at 0.28 mM | Desmodium uncinatum | |
additional information | overview: phenylpropanoids | Desmodium uncinatum | |
NADP+ | 50% inhibition at 0.5 mM; slight inhibition | Desmodium uncinatum | |
pelargonidin | 55% inhibition at 0.006 mM | Desmodium uncinatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NADPH | pH 7.0, 30°C | Desmodium uncinatum | |
0.005 | - |
3,4-cis-leucodelphinidin | pH 7.0, 30°C | Desmodium uncinatum | |
0.006 | - |
2,3-trans-3,4-cis-leucocyanidin | pH 7.0, 30°C | Desmodium uncinatum | |
0.006 | - |
3,4-cis-leucoanthocyanidin | pH 7.0, 30°C | Desmodium uncinatum | |
0.026 | - |
3,4-cis-leucopelargonidin | pH 7.0, 30°C | Desmodium uncinatum | |
0.06 | - |
2,3-trans-3,4-cis-leucocyanidin | pH 7.0, 30°C | Desmodium uncinatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Desmodium uncinatum |
45000 | - |
1 * 45000, native enzyme, SDS-PAGE | Desmodium uncinatum |
52000 | - |
gel filtration | Desmodium uncinatum |
52000 | - |
native enzyme, gel filtration | Desmodium uncinatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADPH | Desmodium uncinatum | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? | |
3,4-cis-leucoanthocyanidin + NADPH | Desmodium uncinatum | - |
2,3-trans-catechin + NADP+ + H2O | no formation of 2,3-cis-epicatechin | ? | |
additional information | Desmodium uncinatum | enzyme is involved in the biosynthesis of condensed tannins or proanthocyanidins 3,4-cis-leucoanthocyanidin, first committed step, anthocyanidin pathway overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desmodium uncinatum | - |
- |
- |
Desmodium uncinatum | Q84V83 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme to near homogeneity from leaves, about 48500fold | Desmodium uncinatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Desmodium uncinatum | - |
leaf | small unexpanded leaflets | Desmodium uncinatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Desmodium uncinatum |
8.7 | - |
purified native enzyme, substrate 2,3-trans-3,4-cis-leucoanthocyanidin | Desmodium uncinatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-trans-3,4-cis-leucocyanidin + NADH + H+ | 30% activity compared to NADPH | Desmodium uncinatum | (2R,3S)-catechin + NAD+ + H2O | - |
r | |
2,3-trans-3,4-cis-leucocyanidin + NADPH | - |
Desmodium uncinatum | (2R,3S)-catechin + NADP+ + H2O | - |
? | |
3,4-cis-leucoanthocyanidin + NADPH | - |
Desmodium uncinatum | 2,3-trans-catechin + NADP+ + H2O | no formation of 2,3-cis-epicatechin | ? | |
3,4-cis-leucodelphinidin + NADPH | - |
Desmodium uncinatum | ? + NADP+ + H2O | - |
? | |
3,4-cis-leucodelphinidin + NADPH | - |
Desmodium uncinatum | 2,3-trans-gallocatechin + NADP+ + H2O | - |
? | |
3,4-cis-leucopelargonidin + NADPH | - |
Desmodium uncinatum | ? + NADP+ + H2O | - |
? | |
3,4-cis-leucopelargonidin + NADPH | - |
Desmodium uncinatum | 2,3-trans-afzelechin + NADP+ + H2O | - |
? | |
additional information | enzyme is involved in the biosynthesis of condensed tannins or proanthocyanidins 3,4-cis-leucoanthocyanidin, first committed step, anthocyanidin pathway overview | Desmodium uncinatum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Desmodium uncinatum |
monomer | 1 * 45000, native enzyme, SDS-PAGE | Desmodium uncinatum |
Synonyms | Comment | Organism |
---|---|---|
LAR | - |
Desmodium uncinatum |
More | enzyme is part of the isoflavone reductase group of the reductase-epimerase-dehydrogenase family of enzymes | Desmodium uncinatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Desmodium uncinatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Desmodium uncinatum |
7 | - |
broad optimum | Desmodium uncinatum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.8 | 95% activity at pH 6.2 and pH 7.8 | Desmodium uncinatum |
6.2 | 7.8 | 95% of maximal activity at pH 76.2 and pH 7.8 | Desmodium uncinatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 30% the rate of NADPH | Desmodium uncinatum | |
NADPH | - |
Desmodium uncinatum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Desmodium uncinatum |