Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.3 extracted from

  • Tanner, G.J.; Francki, K.T.; Abrahams, S.; Watson, J.M.; Larkin, P.J.; Ashton, A.R.
    Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanisdin reductase and molecular cloning of its own cDNA (2003), J. Biol. Chem., 278, 31647-31656.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Desmodium uncinatum
determination of DNA and amino acid sequences, expression in Escherichia coli strain XL-1 Blue, Nicotiana tabacum, and Trifolium repens, plant transformations via Agrobacterium tumefaciens infection system Desmodium uncinatum

Crystallization (Commentary)

Crystallization (Comment) Organism
near homogeneity Desmodium uncinatum

Inhibitors

Inhibitors Comment Organism Structure
2,3-cis-flavan-3,4-diol slight inhibition Desmodium uncinatum
2,3-cis-flavan-3-ol product inhibition Desmodium uncinatum
2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxychromenium 48% inhibition at 0.006 mM Desmodium uncinatum
3,4-trans-leucocyanidin 50% inhibition at 0.12 mM Desmodium uncinatum
3,4-trans-leucopelargonidin 50% inhibition at 0.46 mM Desmodium uncinatum
afzelechin 50% inhibition at 0.014 mM Desmodium uncinatum
catechin 50% inhibition at 0.012 mM Desmodium uncinatum
delphinidin 97% inhibition at 0.06 mM Desmodium uncinatum
dihydroquercetin 60% inhibition at 0.01 mM Desmodium uncinatum
epi-gallocatechin 50% inhibition at 1.4 mM Desmodium uncinatum
eriodictyol 53% inhibition at 0.01 mM Desmodium uncinatum
gallocatechin 50% inhibition at 0.28 mM Desmodium uncinatum
additional information overview: phenylpropanoids Desmodium uncinatum
NADP+ 50% inhibition at 0.5 mM; slight inhibition Desmodium uncinatum
pelargonidin 55% inhibition at 0.006 mM Desmodium uncinatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
NADPH pH 7.0, 30°C Desmodium uncinatum
0.005
-
3,4-cis-leucodelphinidin pH 7.0, 30°C Desmodium uncinatum
0.006
-
2,3-trans-3,4-cis-leucocyanidin pH 7.0, 30°C Desmodium uncinatum
0.006
-
3,4-cis-leucoanthocyanidin pH 7.0, 30°C Desmodium uncinatum
0.026
-
3,4-cis-leucopelargonidin pH 7.0, 30°C Desmodium uncinatum
0.06
-
2,3-trans-3,4-cis-leucocyanidin pH 7.0, 30°C Desmodium uncinatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
1 * 45000, SDS-PAGE Desmodium uncinatum
45000
-
1 * 45000, native enzyme, SDS-PAGE Desmodium uncinatum
52000
-
gel filtration Desmodium uncinatum
52000
-
native enzyme, gel filtration Desmodium uncinatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-trans-3,4-cis-leucocyanidin + NADPH Desmodium uncinatum
-
(2R,3S)-catechin + NADP+ + H2O
-
?
3,4-cis-leucoanthocyanidin + NADPH Desmodium uncinatum
-
2,3-trans-catechin + NADP+ + H2O no formation of 2,3-cis-epicatechin ?
additional information Desmodium uncinatum enzyme is involved in the biosynthesis of condensed tannins or proanthocyanidins 3,4-cis-leucoanthocyanidin, first committed step, anthocyanidin pathway overview ?
-
?

Organism

Organism UniProt Comment Textmining
Desmodium uncinatum
-
-
-
Desmodium uncinatum Q84V83
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to near homogeneity from leaves, about 48500fold Desmodium uncinatum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Desmodium uncinatum
-
leaf small unexpanded leaflets Desmodium uncinatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.7
-
-
Desmodium uncinatum
8.7
-
purified native enzyme, substrate 2,3-trans-3,4-cis-leucoanthocyanidin Desmodium uncinatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-trans-3,4-cis-leucocyanidin + NADH + H+ 30% activity compared to NADPH Desmodium uncinatum (2R,3S)-catechin + NAD+ + H2O
-
r
2,3-trans-3,4-cis-leucocyanidin + NADPH
-
Desmodium uncinatum (2R,3S)-catechin + NADP+ + H2O
-
?
3,4-cis-leucoanthocyanidin + NADPH
-
Desmodium uncinatum 2,3-trans-catechin + NADP+ + H2O no formation of 2,3-cis-epicatechin ?
3,4-cis-leucodelphinidin + NADPH
-
Desmodium uncinatum ? + NADP+ + H2O
-
?
3,4-cis-leucodelphinidin + NADPH
-
Desmodium uncinatum 2,3-trans-gallocatechin + NADP+ + H2O
-
?
3,4-cis-leucopelargonidin + NADPH
-
Desmodium uncinatum ? + NADP+ + H2O
-
?
3,4-cis-leucopelargonidin + NADPH
-
Desmodium uncinatum 2,3-trans-afzelechin + NADP+ + H2O
-
?
additional information enzyme is involved in the biosynthesis of condensed tannins or proanthocyanidins 3,4-cis-leucoanthocyanidin, first committed step, anthocyanidin pathway overview Desmodium uncinatum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Desmodium uncinatum
monomer 1 * 45000, native enzyme, SDS-PAGE Desmodium uncinatum

Synonyms

Synonyms Comment Organism
LAR
-
Desmodium uncinatum
More enzyme is part of the isoflavone reductase group of the reductase-epimerase-dehydrogenase family of enzymes Desmodium uncinatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Desmodium uncinatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Desmodium uncinatum
7
-
broad optimum Desmodium uncinatum

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.8 95% activity at pH 6.2 and pH 7.8 Desmodium uncinatum
6.2 7.8 95% of maximal activity at pH 76.2 and pH 7.8 Desmodium uncinatum

Cofactor

Cofactor Comment Organism Structure
NADH 30% the rate of NADPH Desmodium uncinatum
NADPH
-
Desmodium uncinatum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Desmodium uncinatum