Any feedback?
Literature summary for 1.17.1.10 extracted from
- Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein (1983), J. Biol. Chem., 258, 1826-1832.
General Stability
| General Stability | Organism |
|---|---|
| azide stabilizes during purification | Moorella thermoacetica |
| dithionite stabilizes during purification | Moorella thermoacetica |
| glycerol stabilizes during purification | Moorella thermoacetica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.109 | - |
formate | - |
Moorella thermoacetica |  |
| 0.117 | - |
NADP+ | - |
Moorella thermoacetica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 36 mol of iron per mol of enzyme | Moorella thermoacetica | |
| selenium | 2 mol of selenium per mol of enzyme, resides in the alpha-subunit | Moorella thermoacetica | |
| Tungsten | 2 mol of tungsten per mol of enzyme | Moorella thermoacetica | |
| Tungsten | may exist as tungsten cofactor | Moorella thermoacetica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
| 96000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
| 340000 | - |
gel filtration | Moorella thermoacetica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| extremely oxygen-sensitive | Moorella thermoacetica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1050 | - |
- |
Moorella thermoacetica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, anaerobic conditions, glycerol, ammonium sulfate, 45 days, 35% loss of activity | Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + NADPH | enzyme also catalyzes: 1. reversible electron transfer between methyl viologen and NADPH, 2. reduction of FMN or FAD with NADPH (caused by a contaminating protein) | Moorella thermoacetica | formate + NADP+ | - |
? | |
| CO2 + NADPH | reduction of FMN or FAD with NADPH caused by a contaminating protein | Moorella thermoacetica | formate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Moorella thermoacetica | |
| NADPH | - |
Moorella thermoacetica | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
