Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl compounds | required for activity, e.g. dithiothreitol, cysteine, glutathione, thioglycolate | Moorella thermoacetica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | sodium azide and NADP+ protect | Moorella thermoacetica | |
azide | competitive inhibitor | Moorella thermoacetica | |
cyanide | 50% inhibition at 0.01 mM, 100% inhibition at 10 mM | Moorella thermoacetica | |
EDTA | 50% inhibition at 25 mM, 100% inhibition at 100 mM | Moorella thermoacetica | |
hypophosphite | competitive inhibitor | Moorella thermoacetica | |
mercaptoethanol | - |
Moorella thermoacetica | |
additional information | enzyme is rapidly inactivated by oxygen: 93% loss of activity within 2 min | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | - |
Moorella thermoacetica | |
0.083 | - |
formate | cosubstrate methyl viologen | Moorella thermoacetica | |
0.11 | - |
NADP+ | - |
Moorella thermoacetica | |
0.23 | - |
formate | cosubstrate NADP+ | Moorella thermoacetica | |
2.35 | - |
reduced methyl viologen | - |
Moorella thermoacetica | |
11 | - |
CO2 | - |
Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | - |
Moorella thermoacetica | |
selenium | - |
Moorella thermoacetica | |
Tungsten | - |
Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | 300000 | gel filtration | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADPH | Moorella thermoacetica | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | formate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Oxidation Stability | Organism |
---|---|
rapid inactivation by air, thiol-iron complexes and formate stabilize | Moorella thermoacetica |
Purification (Comment) | Organism |
---|---|
partial | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.6 | - |
- |
Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADPH | enzyme also catalyzes: 3. oxidation of NADPH with O2 | Moorella thermoacetica | formate + NADP+ | - |
r | |
CO2 + NADPH | also reacts with: methyl viologen, benzyl viologen | Moorella thermoacetica | formate + NADP+ | - |
r | |
CO2 + NADPH | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | Moorella thermoacetica | formate + NADP+ | - |
? | |
formate + reduced methyl viologen | - |
Moorella thermoacetica | CO2 + oxidized methyl viologen | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
formate + NADP+ | Moorella thermoacetica |
85 | - |
formate + methyl viologen | Moorella thermoacetica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
low activity below | Moorella thermoacetica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
3 h, slight loss of activity | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
broad, CO2 reduction | Moorella thermoacetica |
7 | 9.5 | formate oxidation | Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Moorella thermoacetica | |
NADPH | - |
Moorella thermoacetica |