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Literature summary for 1.16.3.2 extracted from

  • Sanchuki, H.B.; Valdameri, G.; Moure, V.R.; Rodriguez, J.A.; Pedrosa, F.O.; Souza, E.M.; Korolik, V.; Ribeiro, R.R.; Huergo, L.F.
    Conserved histidine residues at the ferroxidase centre of the Campylobacter jejuni Dps protein are not strictly required for metal binding and oxidation (2016), Microbiology, 162, 156-163 .
    View publication on PubMed
Search for more literature for 1.16.3.2

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) by thermal treatment and two chromatographic steps Campylobacter jejuni subsp. jejuni

Protein Variants

Protein Variants Comment Organism
H25G site-directed mutagenesis, the mutant is able to accumulate iron in its inner cavity similar to the wild-type enzyme Campylobacter jejuni subsp. jejuni
H25G/H37G site-directed mutagenesis, the mutant shows reduced activity to accumulate iron in its inner cavity compared to the wild-type enzyme Campylobacter jejuni subsp. jejuni
H37G site-directed mutagenesis, the mutant is able to accumulate iron in its inner cavity similar to the wild-type enzyme Campylobacter jejuni subsp. jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic profile of wild-type and mutant enzymes, overview Campylobacter jejuni subsp. jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a non-heme iron protein Campylobacter jejuni subsp. jejuni
Mn2+ can bind to the enzyme, structure and kinetic analysis Campylobacter jejuni subsp. jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Fe(II) + H2O2 + 2 H2O Campylobacter jejuni subsp. jejuni
-
 2 [FeO(OH)] + 4 H+
-
 ?
2 Fe(II) + H2O2 + 2 H2O Campylobacter jejuni subsp. jejuni ATCC 700819
-
 2 [FeO(OH)] + 4 H+
-
 ?
2 Fe(II) + O2 + 4 H2O Campylobacter jejuni subsp. jejuni
-
 2 [FeO(OH)] + 4 H+ + H2O2
-
 ?
2 Fe(II) + O2 + 4 H2O Campylobacter jejuni subsp. jejuni ATCC 700819
-
 2 [FeO(OH)] + 4 H+ + H2O2
-
 ?
4 Fe(II) + O2 + 6 H2O Campylobacter jejuni subsp. jejuni
-
 4 [FeO(OH)] + 8 H+
-
 ?
4 Fe(II) + O2 + 6 H2O Campylobacter jejuni subsp. jejuni ATCC 700819
-
 4 [FeO(OH)] + 8 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni subsp. jejuni Q0P891
-
-
Campylobacter jejuni subsp. jejuni ATCC 700819 Q0P891
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(II) + H2O2 + 2 H2O
-
 Campylobacter jejuni subsp. jejuni 2 [FeO(OH)] + 4 H+
-
 ?
2 Fe(II) + H2O2 + 2 H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 2 [FeO(OH)] + 4 H+
-
 ?
2 Fe(II) + O2 + 4 H2O
-
 Campylobacter jejuni subsp. jejuni 2 [FeO(OH)] + 4 H+ + H2O2
-
 ?
2 Fe(II) + O2 + 4 H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 2 [FeO(OH)] + 4 H+ + H2O2
-
 ?
4 Fe(II) + O2 + 6 H2O
-
 Campylobacter jejuni subsp. jejuni 4 [FeO(OH)] + 8 H+
-
 ?
4 Fe(II) + O2 + 6 H2O overall reaction Campylobacter jejuni subsp. jejuni 4 [FeO(OH)] + 8 H+
-
 ?
4 Fe(II) + O2 + 6 H2O
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 4 [FeO(OH)] + 8 H+
-
 ?
4 Fe(II) + O2 + 6 H2O overall reaction Campylobacter jejuni subsp. jejuni ATCC 700819 4 [FeO(OH)] + 8 H+
-
 ?
additional information the formation of the iron hydroxide core is monitored spectrophotometrically at 305 nm. The enzyme is able to bind DNA, DNA-binding analysis fro wild-type and mutant enzymes, overview Campylobacter jejuni subsp. jejuni ?
-
 ?
additional information the formation of the iron hydroxide core is monitored spectrophotometrically at 305 nm. The enzyme is able to bind DNA, DNA-binding analysis fro wild-type and mutant enzymes, overview Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer gel filtration and dynamic laser scaterring Campylobacter jejuni subsp. jejuni

Synonyms

Synonyms Comment Organism
CjDps
-
 Campylobacter jejuni subsp. jejuni
DNA-binding protein from starved cells
-
 Campylobacter jejuni subsp. jejuni
Dps protein
-
 Campylobacter jejuni subsp. jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22 25 assay at Campylobacter jejuni subsp. jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7 assay at Campylobacter jejuni subsp. jejuni

General Information

General Information Comment Organism
evolution Dps (DNA-binding protein from starved cells) belongs to a subfamily of ferritins. The presence of a ferroxidase centre, composed of highly conserved residues, is a signature of this protein family Campylobacter jejuni subsp. jejuni
additional information two conserved histidine residues, H25 and H37, located at the ferroxidase centre of the Campylobacter jejuni Dps (DNA-binding protein from starved cells) protein, are not strictly required for metal binding and oxidation. The archetypical function of Dps seems to be DNA protection against hydroxyl radicals that are produced when Fe2+ and H2O2 combine Campylobacter jejuni subsp. jejuni
physiological function Dps proteins are widely distributed in being required for survival during stressful conditions such as nutrient starvation, thermal stress and oxidative conditions, and inside biofilm. Campylobacter jejuni needs to be able to counteract various types of environmental stress during colonization, for example extreme pH and low availability of trace metals such as iron. Dps proteins can store iron atoms inside the dodecamer. Campylobacter jejuni Dps is able to bind DNA, and the DNA-binding activity is stimulated by Fe2+ (at room temperature in Bis-Tris, pH 6.0), overview Campylobacter jejuni subsp. jejuni