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Literature summary for 1.16.3.1 extracted from

  • Noyer, M.; Dwulet, F.E.; Hao, Y.L.; Putnam, F.W.
    Purification and characterization of undegraded human ceruloplasmin (1980), Anal. Biochem., 102, 450-458.
    View publication on PubMed
Search for more literature for 1.16.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 N,N'-dimethyl-p-phenylenediamine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18650
-
 1 * 18650 + 1 * 50000 + 1 * 70000, limited proteolysis Homo sapiens
50000
-
 1 * 18650 + 1 * 50000 + 1 * 70000, limited proteolysis Homo sapiens
70000
-
 1 * 18650 + 1 * 50000 + 1 * 70000, limited proteolysis Homo sapiens
132000
-
 amino acid sequence Homo sapiens
135000
-
 undegraded single-chain protein, gel filtration, analytical SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fe2+ + H+ + O2 Homo sapiens
-
 Fe3+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Homo sapiens
-
plasma
-
 Homo sapiens
-
serum
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, 0.5 M phosphate buffer, pH 6.9, concentration of approximately 2.5% without noticeable loss of blue colour during 1 month Homo sapiens
-20°C, frozen in liquid nitrogen 0.2% enzyme solution in 0.015 M phosphate buffer, pH 6.9, containing 0.1 M NaCl loses its blue colour completely after 2 weeks storage Homo sapiens
4°C, 0.2% enzyme solution in 0.015 M phosphate buffer, pH 6.9, containing 0.1 M NaCl: the A610/A280 ratio is reduced by approximately 10% in 1 month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe2+ + H+ + O2
-
 Homo sapiens Fe3+ + H2O
-
 ?
N,N'-dimethyl-p-phenylenediamine + Fe2+ + O2
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 18650 + 1 * 50000 + 1 * 70000, limited proteolysis Homo sapiens

Synonyms

Synonyms Comment Organism
blue copper oxidase
-
 Homo sapiens