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Literature summary for 1.16.1.9 extracted from

  • Sagi-Kazar, M.; Zelenyanszki, H.; Mueller, B.; Cseh, B.; Gyuris, B.; Farkas, S.Z.; Fodor, F.; Toth, B.; Kovacs, B.; Koncz, A.; Visnovitz, T.; Buzas, E.I.; Bankuti, B.; Banati, F.; Szenthe, K.; Solti, A.
    Supraoptimal iron nutrition of Brassica napus plants suppresses the iron uptake of chloroplasts by down-regulating chloroplast ferric chelate reductase (2021), Front. Plant Sci., 12, 658987.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Phaseolus vulgaris leaf chloroplasts Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02207
-
 Fe(III)-EDTA at optimal iron nutrition, pH and temperature not specified in the publication Brassica napus
0.02346
-
 Fe(III)-EDTA at iron deficiency, pH and temperature not specified in the publication Brassica napus
0.03022
-
 Fe(III)-citrate at suboptimal iron nutrition, pH and temperature not specified in the publication Brassica napus
0.04637
-
 Fe(III)-citrate at iron deficiency, pH and temperature not specified in the publication Brassica napus
0.047
-
 Fe(III)-citrate at optimal iron nutrition, pH and temperature not specified in the publication Brassica napus
0.05252
-
 Fe(III)-EDTA at suboptimal iron nutrition, pH and temperature not specified in the publication Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast inner chloroplast envelope membrane Brassica napus 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM used in assay conditions Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 cultivar DK Exquisite
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(III)-citrate + NADPH
-
 Brassica napus 2 Fe(II)-citrate + NADP+ + H+
-
 ?
2 Fe(III)-EDTA + NADPH
-
 Brassica napus 2 Fe(II)-EDTA + NADP+ + H+
-
 ?

Synonyms

Synonyms Comment Organism
FCR
-
 Brassica napus
ferric reductase oxidase 7
-
 Brassica napus
FRO7
-
 Brassica napus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.5
-
 melting temperature Brassica napus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Brassica napus
NADPH
-
 Brassica napus

Expression

Organism Comment Expression
Brassica napus iron deficiency does not increase isoform FRO7 expression additional information