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Literature summary for 1.16.1.9 extracted from

  • Umezawa, S.; Akao, H.; Kubota, M.; Kino, K.
    Chemoenzymatic oxygenation method for sesquiterpenoid synthesis based on Fe-chelate and ferric-chelate reductase (2020), Biosci. Biotechnol. Biochem., 84, 780-788.
    View publication on PubMed
Search for more literature for 1.16.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0035
-
 with Fe(III)-cyclam as substrate, at pH 7.0 and 30°C Escherichia coli
0.0204
-
 with Fe(III)-diethylenetriaminepentaacetate as substrate, at pH 7.0 and 30°C Escherichia coli
0.0294
-
 with Fe(III)-(citrate)2 as substrate, at pH 7.0 and 30°C Escherichia coli
0.0333
-
 with Fe(III)-tris(2-pyridylmethyl)amine as substrate, at pH 7.0 and 30°C Escherichia coli
0.0393
-
 with Fe(III)-EDTA as substrate, at pH 7.0 and 30°C Escherichia coli
0.0408
-
 with Fe(III)-(1,10-phenanthroline)3 as substrate, at pH 7.0 and 30°C Escherichia coli
0.0454
-
 with Fe(III)-(catechol)2 as substrate, at pH 7.0 and 30°C Escherichia coli
0.047
-
 with Fe(III)-nitrilotriacetic acid as substrate, at pH 7.0 and 30°C Escherichia coli
0.0907
-
 with Fe(III)-(L-DOPA)2 as substrate, at pH 7.0 and 30°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(III)-(1,10-phenanthroline)3 + NADPH
-
 Escherichia coli 2 Fe(II)-(1,10-phenanthroline)3 + NADP+ + H+
-
 ?
2 Fe(III)-(catechol)2 + NADPH
-
 Escherichia coli 2 Fe(II)-(catechol)2 + NADP+ + H+
-
 ?
2 Fe(III)-(citrate)2 + NADPH
-
 Escherichia coli 2 Fe(II)-(citrate)2 + NADP+ + H+
-
 ?
2 Fe(III)-(L-DOPA)2 + NADPH highest specific activity Escherichia coli 2 Fe(II)-(L-DOPA)2 + NADP+ + H+
-
 ?
2 Fe(III)-cyclam + NADPH lowest specific activity Escherichia coli 2 Fe(II)-cyclam + NADP+ + H+
-
 ?
2 Fe(III)-diethylenetriaminepentaacetate + NADPH
-
 Escherichia coli 2 Fe(II)-diethylenetriaminepentaacetate + NADP+ + H+
-
 ?
2 Fe(III)-EDTA + NADPH
-
 Escherichia coli 2 Fe(II)-EDTA + NADP+ + H+
-
 ?
2 Fe(III)-nitrilotriacetic acid + NADPH
-
 Escherichia coli 2 Fe(II)-nitrilotriacetic acid + NADP+ + H+
-
 ?
2 Fe(III)-tris(2-pyridylmethyl)amine + NADPH
-
 Escherichia coli 2 Fe(II)-tris(2-pyridylmethyl)amine + NADP+ + H+
-
 ?

Synonyms

Synonyms Comment Organism
YqjH
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Escherichia coli