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Literature summary for 1.16.1.9 extracted from

  • Wang, S.; Wu, Y.; Outten, F.
    Fur and the novel regulator Yqji control transcription of the ferric reductase gene yqjh in Escherichia coli (2011), J. Bacteriol., 193, 563-574.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 Fe(III)-EDTA at pH 7.5 and 37°C Escherichia coli
0.043
-
 NADPH at pH 7.5 and 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MG1655
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap DEAE column chromatography, Superdex 75 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
 using ferric enterobactin as substrate, at pH 7.5 and 37°C Escherichia coli
0.022
-
 using Fe(III)-EDTA as substrate, at pH 7.5 and 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe(III)-EDTA + NADPH + H+
-
 Escherichia coli Fe(II) + EDTA + NADP+
-
 ?
Fe(III)-EDTA + NADPH + H+
-
 Escherichia coli MG1655 Fe(II) + EDTA + NADP+
-
 ?
ferric enterobactin + NADPH + H+ weak activity Escherichia coli ?
-
 ?
ferric enterobactin + NADPH + H+ weak activity Escherichia coli MG1655 ?
-
 ?
additional information the enzyme shows no ferric reductase activity toward ferric chloride Escherichia coli ?
-
 ?
additional information the enzyme shows no ferric reductase activity toward ferric chloride Escherichia coli MG1655 ?
-
 ?

Synonyms

Synonyms Comment Organism
ferric reductase
-
 Escherichia coli
YqjH
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme uses FADH2/FAD as a covalently bound cofactor rather than a substrate Escherichia coli
NADPH dependent on Escherichia coli

Expression

Organism Comment Expression
Escherichia coli addition of Fe2+ decreases yqjH expression starting at 100 nM, with a maximum repression of 4fold occurring at 0.01 mM Fe2+. Addition of CoCl2 concentrations of more than 500 nM also repress yqjH expression by about 50% down
Escherichia coli YqjI-dependent transcriptional repression is reduced when cells are exposed to elevated nickel levels, resulting in increased expression of yqjH. Upon addition of NiCl2, yqjH expression increases starting at 100 nM NiCl2, with maximum induction by 0.001 mM NiCl2 (42%). Deletion of yqjI leads to constitutive expression levels of yqjH that are 30fold higher than wild type basal expression levels up

General Information

General Information Comment Organism
malfunction deletion of yqjH increases nickel toxicity Escherichia coli
physiological function the enzyme is required for iron homeostasis in Escherichia coli Escherichia coli