Cloned (Comment) | Organism |
---|---|
expression of full-length enzyme and flavin-binding domain as GST-tagged proteins | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | lack of control on the thermodynamics and kinetics of electron transfer in MSR, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | Homo sapiens | MSR is a NADPH-dependent diflavin oxidoreductase required for the reductive regeneration of catalytically inert cob(II)alamin to cob(I)alamin, complex formation between the substrate's activation domain and MSR, and the substrate's activation domain and the isolated FMN-binding domain of MSR. Weshow that the MS activation domain interacts directly with the FMN-binding domain of MSR, overview | [methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged full-length enzyme and flavin-binding domain by glutathione affinity and anion exchange chromatography, followed by ultrafiltration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | MSR is a NADPH-dependent diflavin oxidoreductase required for the reductive regeneration of catalytically inert cob(II)alamin to cob(I)alamin, complex formation between the substrate's activation domain and MSR, and the substrate's activation domain and the isolated FMN-binding domain of MSR. Weshow that the MS activation domain interacts directly with the FMN-binding domain of MSR, overview | Homo sapiens | [methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r | |
[methionine synthase]-methylcob(I)alamin + S-adenosylhomocysteine + NADP+ | interaction of the enzyme with the substrate enzyme methionine synthase via the C-terminal domain involves the residues K987 and K1071, interaction with substrate mutants K987T and K1071T is affected, structure-function relationship, overview | Homo sapiens | [methionine synthase]-cob(II)alamin + NADPH + S-adenosyl-L-methionine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Methionine synthase reductase | - |
Homo sapiens |
MSR | - |
Homo sapiens |
NADPH-dependent diflavin oxidoreductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | midpoint reduction potential | Homo sapiens | |
FMN | midpoint reduction potential | Homo sapiens | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |