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Literature summary for 1.16.1.10 extracted from

  • Vadas, A.; Monbouquette, H.G.; Johnson, E.; Schroeder, I.
    Identification and characterization of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus (1999), J. Biol. Chem., 274, 36715-36721.
    View publication on PubMed
Search for more literature for 1.16.1.10

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 NADH pH 7.0, 85°C Archaeoglobus fulgidus
0.066
-
 Fe(III)-EDTA pH 7.0, 85°C, cosubstrate: NADPH Archaeoglobus fulgidus
0.08
-
 NADPH pH 7.0, 85°C Archaeoglobus fulgidus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information high cellular abundance, 0.75% of the total soluble protein Archaeoglobus fulgidus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require Mg2+ for optimal activity Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 2 * 18000, SDS-PAGE Archaeoglobus fulgidus
18659
-
 2 * 18659, calculated from sequence Archaeoglobus fulgidus
40000
-
 gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29428
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3503
-
 pH 7.0, 85°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(III)-EDTA + NADH no activity is obtained with uncomplexed Fe3+, i.e. FeCl3 and Fe(OH)3, with the menaquinone analog dimethylnaphthoquinone or with Fe2+-EDTA. The enzyme does not reduce EDTA-chelated Ag+ and Cu2+. With Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor Archaeoglobus fulgidus 2 Fe(II)-EDTA + NAD+ + H+
-
 ?
2 Fe(III)-EDTA + NADPH no activity is obtained with uncomplexed Fe3+, i.e. FeCl3 and Fe(OH)3, with the menaquinone analog dimethylnaphthoquinone or with Fe2+-EDTA. The enzyme does not reduce EDTA-chelated Ag+ and Cu2+. With Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor Archaeoglobus fulgidus 2 Fe(II)-EDTA + NADP+ + H+
-
 ?
additional information the ferric reductase also functions as an NAD(P)H:flavin oxidoreductase Archaeoglobus fulgidus ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 18000, SDS-PAGE Archaeoglobus fulgidus
homodimer 2 * 18659, calculated from sequence Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
AF0830
-
 Archaeoglobus fulgidus
ferric reductase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Archaeoglobus fulgidus
88
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 50°C: about 50% of maximal activity, 100°C: about 50% of maximal activity Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 40% of maximal activity, pH 8.0: about 60% of maximal activity Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein, enzyme bound flavin is involved in catalysis, contains 1.4 FMN per monomer, non-covalently bound to the enzyme Archaeoglobus fulgidus
NADH with Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor Archaeoglobus fulgidus
NADPH with Fe3+-EDTA as the electron acceptor, the purified enzyme exhibits a slightly higher affinity and Vmax for NADH than for NADPH as the electron donor Archaeoglobus fulgidus

pI Value

Organism Comment pI Value Maximum pI Value
Archaeoglobus fulgidus calculated from sequence
-
 5.84