Literature summary for 1.16.1.1 extracted from

Zhang, W.; Chen, L.; Liu, D.
Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction (2012), Appl. Microbiol. Biotechnol., 93, 1305-1314.

Search for more literature for 1.16.1.1

Application

Application	Comment	Organism
environmental protection	the organism can potentially be used for bioremediation in marine environments	Pseudomonas putida

Cloned(Commentary)

Cloned (Comment)	Organism
gene merA, mer operon located on Tn5041, DNA and amino acid sequence determination and analysis, chromosomal localization, real-time PCR enzyme expression analysis	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the organism is highly resistant to the antibiotics ampicillin, kanamycin, chloramphenicol, and tetracycline	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the organism is resistant to CdCl2, CoCl2, CrCl3, CuCl2, PbCl2, ZnSO4, and to 0.28 mM HgCl2	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Hg + NADP+ + H+	Pseudomonas putida	the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to	Hg2+ + NADPH	-	r
Hg + NADP+ + H+	Pseudomonas putida SP1	the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to	Hg2+ + NADPH	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	marine-isolated mercury-resistant strain, isolated from seawater collected from Yantai coastal zone in Shandong Province, China, gene merA encoded in the mer operon	-
Pseudomonas putida SP1	-	marine-isolated mercury-resistant strain, isolated from seawater collected from Yantai coastal zone in Shandong Province, China, gene merA encoded in the mer operon	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the low virulence strain is part of the biofilm. Optimal pH for the growth and enzyme activity of strain SP1 in presence of HgCl2 is pH 8.0-9.0, whereas optimal pH for expression of merA is pH 5.0	Pseudomonas putida	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hg + NADP+ + H+	-	Pseudomonas putida	Hg2+ + NADPH	-	r
Hg + NADP+ + H+	the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to	Pseudomonas putida	Hg2+ + NADPH	-	r
Hg + NADP+ + H+	-	Pseudomonas putida SP1	Hg2+ + NADPH	-	r
Hg + NADP+ + H+	the enzmye reduces reactive Hg2+ to volatile and relatively inert monoatomic Hg0 vapor. Pseudomonas putida SP1 is able to volatilize almost 100% of the total mercury it is exposed to	Pseudomonas putida SP1	Hg2+ + NADPH	-	r

Synonyms

Synonyms	Comment	Organism
mercuric reductase	-	Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
28	-	assay at	Pseudomonas putida

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Pseudomonas putida

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	high activity within that range	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)