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Literature summary for 1.16.1.1 extracted from

  • Schneider, M.; Deckwer, W.
    Kinetics of mercury reduction by Serratia marcescens mercuric reductase expressed by Pseudomonas putida strains (2005), Eng. Life Sci., 5, 415-424.
No PubMed abstract available
Search for more literature for 1.16.1.1

Protein Variants

Protein Variants Comment Organism
additional information constitutive expression of enzyme in Pseudomonas putida results in a broad spectrum mercury resistance Serratia marcescens

Inhibitors

Inhibitors Comment Organism Structure
Cl- inhibitory above 0.1 M, complete inhibition at 1 M Serratia marcescens
Hg2+ pronounced substrate inhibition Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 Hg2+ pH 7.2, 30°C, detailed kinetics Serratia marcescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 3 * 65000, SDS-PAGE Serratia marcescens
190000
-
 gel filtration Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
 expressed by Pseudomonas putida
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hg2+ + NADPH
-
 Serratia marcescens Hg + NADP+ + H+
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 65000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.8
-
 Serratia marcescens

pI Value

Organism Comment pI Value Maximum pI Value
Serratia marcescens isoelectric focusing
-
 5.1