Application | Comment | Organism |
---|---|---|
environmental protection | application of the immobilized mercuric reductase for continuous treatment of Hg(II)-containing water in a fixed bed reactor | Pseudomonas putida |
General Stability | Organism |
---|---|
operational stability: after 1.5 h decline of activity up to 20% | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
KT2442::mer-73 | - |
Pseudomonas putida KT2442::mer-73 | - |
KT2442::mer-73 | - |
Storage Stability | Organism |
---|---|
4°C, 50 mM potassium phosphate buffer, pH 7.2, 0.5 mM EDTA, 1% 2-mercaptoethanol, half-life of soluble enzyme is 3 weeks, immobilized enzyme shows large decline at the beginning and almost no further decrease of activity after 3 weeks | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg2+ + NADPH | - |
Pseudomonas putida | Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | - |
Pseudomonas putida KT2442::mer-73 | Hg + NADP+ + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas putida |