Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | strong inhibition | Stemona tuberosa | |
H2O2 | strong inhibition | Stemona tuberosa | |
Hg2+ | 35% inhibition at 0.1 mM, complete inhibition at 5 mM | Stemona tuberosa | |
Mg2+ | 25% inhibition at 5 mM | Stemona tuberosa | |
Mn2+ | 35% inhibition at 5 mM | Stemona tuberosa | |
SDS | 19% inhibition at 0.1 mM, complete inhibition at 0.5 mM | Stemona tuberosa | |
Zn2+ | 12% inhibition at 5 mM | Stemona tuberosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates slightly by 10% at 5 mM | Stemona tuberosa | |
Fe2+ | activates slightly by 10% at 5 mM | Stemona tuberosa | |
additional information | no effect on enzyme activity by Cu2+ at 0.1-5 mM | Stemona tuberosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Stemona tuberosa | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stemona tuberosa | - |
from the Chatujak market, Bangkok, Thailand, in June 2009 | - |
Purification (Comment) | Organism |
---|---|
native enzyme from tubers/roots 3fold by ammonium sulfate fractionation and anion exchange chromatography | Stemona tuberosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Stemona tuberosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83.3 | - |
purified native enzyme, pH 7.2, 37°C | Stemona tuberosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Stemona tuberosa | O2 + H2O2 | - |
? | |
additional information | enzyme activity determination with riboflavin and nitroblue tetrazolium (NBT), kinetics | Stemona tuberosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 17600+ 1 * 31500, SDS-PAGE | Stemona tuberosa |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Stemona tuberosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Stemona tuberosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | purified native enzyme, stable for 120 min | Stemona tuberosa |
60 | - |
purified native enzyme, loss of 50% activity after 120 min | Stemona tuberosa |
70 | - |
purified native enzyme, loss of 70% activity after 120 min | Stemona tuberosa |
80 | - |
purified native enzyme, inactivation after 90 min | Stemona tuberosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Stemona tuberosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | purified native enzyme, activitiy determination after 20-120 min, overview | Stemona tuberosa |
General Information | Comment | Organism |
---|---|---|
additional information | tryptic peptide mapping | Stemona tuberosa |
physiological function | superoxide dismutase is an antioxidant enzyme that plays an important role in the removal of harmful superoxide radicals, which are generated by metabolic processes in oxygen-utilizing organisms. Biological activity of purified SOD from the root of Stemona tuberosa on in vitro cell proliferation of skin fibroblast cells from human | Stemona tuberosa |