Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chloroform | the enzyme activity increases by about 20% in glycerol or chloroform, glycerol and chloroform may supply a hydrophobic environment that is conducive to better contact with the substrate | Sonneratia alba | |
glycerol | the enzyme activity increases by about 20% in glycerol or chloroform, glycerol and chloroform may supply a hydrophobic environment that is conducive to better contact with the substrate | Sonneratia alba | |
additional information | enzyme SaCSD1 is degraded rapidly and completely after enzymatic treatment with pepsin. No full-length protein is detectable even after 0.5 min of digestion | Sonneratia alba |
Cloned (Comment) | Organism |
---|---|
gene CSD1, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time quantitative PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami | Sonneratia alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Sonneratia alba | |
H2O2 | - |
Sonneratia alba | |
additional information | the SaCSD1 protein is very susceptive to pepsin digestion | Sonneratia alba | |
NaCl | activity of SaCSD1 decreases with the increase of salt concentration | Sonneratia alba | |
phenol | - |
Sonneratia alba | |
SDS | - |
Sonneratia alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sonneratia alba | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu/Zn-SOD | Sonneratia alba | |
Zn2+ | a Cu/Zn-SOD | Sonneratia alba |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Sonneratia alba | - |
O2 + H2O2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
chloroform | the enzyme activity increases by about 20% in glycerol or chloroform | Sonneratia alba |
DMSO | 90% residual activity in DMSO | Sonneratia alba |
Glycerol | the enzyme activity increases by about 20% in glycerol or chloroform | Sonneratia alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sonneratia alba | A0A0U1X631 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the SaCSD1 protein is very susceptive to pepsin digestion | Sonneratia alba |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 3.2fold from Escherichia coli strain Rosetta-gami by nickel affinity chromatography | Sonneratia alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Sonneratia alba | - |
fruit | - |
Sonneratia alba | - |
leaf | - |
Sonneratia alba | - |
additional information | enzyme expression pattern analysis | Sonneratia alba | - |
stem | - |
Sonneratia alba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2200 | - |
purified recombinant His-tagged enzyme, pH 6.0, 25°C | Sonneratia alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Sonneratia alba | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15320, SaCSD1, sequence calculation | Sonneratia alba |
Synonyms | Comment | Organism |
---|---|---|
copper/zinc superoxide dismutase | - |
Sonneratia alba |
SaCSD1 | - |
Sonneratia alba |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | broad optimum | Sonneratia alba |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | high activity range | Sonneratia alba |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | purified recombinant His-tagged enzyme, no significant change in enzyme activity at 25-45°C for up to 1 h, at 75°C, 60% of enzyme activity is retained after 1 h of treatment, suggesting that the SaCSD1 is relatively thermostable | Sonneratia alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Sonneratia alba |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | high activity range | Sonneratia alba |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | purified recombinant His-tagged enzyme, stable at | Sonneratia alba |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sonneratia alba | sequence calculation | - |
5.78 |
Organism | Comment | Expression |
---|---|---|
Sonneratia alba | under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 is downregulated in roots, but upregulated in leaves | down |
Sonneratia alba | under 0.25 M and 0.5 M salt stress, the expression of SaCSD1 is downregulated in roots, but upregulated in leaves | up |