Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.15.1.1 extracted from

  • Ben Khaled, H.; Ktari, N.; Siala, R.; Hmidet, N.; Bayoudh, A.; Nasri, M.; Ghorbel, S.
    A heat-stable Cu/Zn superoxide dismutase from the viscera of sardinelle (Sardinella aurita) purification and biochemical characterization (2014), Biologia (Poland), 69, 1770-1776 .
No PubMed abstract available
Search for more literature for 1.15.1.1

Inhibitors

Inhibitors Comment Organism Structure
H2O2 90% inhibition at 5 mM Sardinella aurita
KCN complete inhibition at 5 mM Sardinella aurita

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a Cu/Zn SOD Sardinella aurita
Zn2+ a Cu/Zn SOD Sardinella aurita

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 native enzyme, gel filtration Sardinella aurita

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 superoxide + 2 H+ Sardinella aurita
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Sardinella aurita
-
 from the fish market of Sfax City, Tunisia
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from sardinelle viscera to homogeneity 7.17fold by heat treatment at 65°C for 15 min, ammonium sulfate fractionation, and gel filtration Sardinella aurita

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
228.19
-
 purified native enzyme, pH 7.0, 37°C Sardinella aurita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 superoxide + 2 H+
-
 Sardinella aurita O2 + H2O2
-
 ?
additional information SOD activity is measured using a method employing xanthine and xanthine oxidase to generate superoxide radicals which react with 2-(4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolium chloride (INT) to form a red formazan dye. The SOD activity is then measured spectrophotometrically by the degree of inhibition of this reaction by means of the decrease in absorbance at 505 nm, 37°C Sardinella aurita ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 20000, native enzyme, SDS-PAGE Sardinella aurita
More the N-terminal sequence of the purified native enzyme is VLKAVCVLKGTGEVT Sardinella aurita

Synonyms

Synonyms Comment Organism
Cu/Zn SOD
-
 Sardinella aurita
Cu/Zn superoxide dismutase
-
 Sardinella aurita
SOD
-
 Sardinella aurita

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Sardinella aurita

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range, profile overview. Relative activities at 25°C and 50°C are about 80% and 73%, respectively, of maximal activity at 40°C Sardinella aurita

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified native enzyme, more than 85% of its initial activity is retained after 1 h of incubation. The enzyme is highly stable at temperatures below 50°C Sardinella aurita
60
-
 purified native enzyme, 45% of its initial activity is retained after 1 h of incubation Sardinella aurita
70
-
 purified native enzyme, 37% of its initial activity is retained after 1 h of incubation Sardinella aurita

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Sardinella aurita

pH Range

pH Minimum pH Maximum Comment Organism
4 11 activity range, profile overview Sardinella aurita

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 at pH 6.0 and pH 9.0, the enzyme retains about 78% and 75%, respectively, of maximal activity after 1 h incubation at 37°C Sardinella aurita

General Information

General Information Comment Organism
physiological function superoxide dismutase (SOD) is an enzyme that protects against oxidative stress from superoxide radicals in living cells Sardinella aurita