Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | 90% inhibition at 5 mM | Sardinella aurita | |
KCN | complete inhibition at 5 mM | Sardinella aurita |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu/Zn SOD | Sardinella aurita | |
Zn2+ | a Cu/Zn SOD | Sardinella aurita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
native enzyme, gel filtration | Sardinella aurita |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Sardinella aurita | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sardinella aurita | - |
from the fish market of Sfax City, Tunisia | - |
Purification (Comment) | Organism |
---|---|
native enzyme from sardinelle viscera to homogeneity 7.17fold by heat treatment at 65°C for 15 min, ammonium sulfate fractionation, and gel filtration | Sardinella aurita |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
228.19 | - |
purified native enzyme, pH 7.0, 37°C | Sardinella aurita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Sardinella aurita | O2 + H2O2 | - |
? | |
additional information | SOD activity is measured using a method employing xanthine and xanthine oxidase to generate superoxide radicals which react with 2-(4-iodophenyl)-3-(4-nitrophenol)-5-phenyltetrazolium chloride (INT) to form a red formazan dye. The SOD activity is then measured spectrophotometrically by the degree of inhibition of this reaction by means of the decrease in absorbance at 505 nm, 37°C | Sardinella aurita | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, native enzyme, SDS-PAGE | Sardinella aurita |
More | the N-terminal sequence of the purified native enzyme is VLKAVCVLKGTGEVT | Sardinella aurita |
Synonyms | Comment | Organism |
---|---|---|
Cu/Zn SOD | - |
Sardinella aurita |
Cu/Zn superoxide dismutase | - |
Sardinella aurita |
SOD | - |
Sardinella aurita |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sardinella aurita |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | activity range, profile overview. Relative activities at 25°C and 50°C are about 80% and 73%, respectively, of maximal activity at 40°C | Sardinella aurita |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified native enzyme, more than 85% of its initial activity is retained after 1 h of incubation. The enzyme is highly stable at temperatures below 50°C | Sardinella aurita |
60 | - |
purified native enzyme, 45% of its initial activity is retained after 1 h of incubation | Sardinella aurita |
70 | - |
purified native enzyme, 37% of its initial activity is retained after 1 h of incubation | Sardinella aurita |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sardinella aurita |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | activity range, profile overview | Sardinella aurita |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | at pH 6.0 and pH 9.0, the enzyme retains about 78% and 75%, respectively, of maximal activity after 1 h incubation at 37°C | Sardinella aurita |
General Information | Comment | Organism |
---|---|---|
physiological function | superoxide dismutase (SOD) is an enzyme that protects against oxidative stress from superoxide radicals in living cells | Sardinella aurita |