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Literature summary for 1.15.1.1 extracted from
- Expression and characterization of a recombinant psychrophilic Cu/Zn superoxide dismutase from Deschampsia antarctica E. Desv. [Poaceae] (2015), Appl. Biochem. Biotechnol., 175, 3287-3296 .
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the highly thermostable enzyme DaSOD might be useful for preventing the oxidation of refrigerated or frozen foods, as well as in the preparation of cosmetic and pharmaceutical products | Deschampsia antarctica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-SI | Deschampsia antarctica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Deschampsia antarctica |  |
| KCN | - |
Deschampsia antarctica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | typical Michaelis-Menten kinetics | Deschampsia antarctica |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a Cu/ZnSOD | Deschampsia antarctica | |
| Zn2+ | a Cu/ZnSOD | Deschampsia antarctica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | Deschampsia antarctica | - |
O2 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deschampsia antarctica | D2DSG2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21-SI by nickel affinity chromatography, dialysis, and ultrafiltration to over 97% purity | Deschampsia antarctica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5818 | - |
purified recombinant enzyme, pH 7.2, 25°C | Deschampsia antarctica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 superoxide + 2 H+ | - |
Deschampsia antarctica | O2 + H2O2 | - |
? | |
| additional information | the enzyme activity assay uses the p-nitro blue tetrazolium chloride (NBT) solution method | Deschampsia antarctica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | enzyme tertiary structural modeling, each monomer contains an eight-barrel chain with seven loops | Deschampsia antarctica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cu/Zn superoxide dismutase | - |
Deschampsia antarctica |
| DaSOD | - |
Deschampsia antarctica |
| SOD | - |
Deschampsia antarctica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Deschampsia antarctica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
DaSOD belongs to a very small group of SODs active at cold temperatures (0°C), and the second of its kind active at -20°C | Deschampsia antarctica |
- |
70 | activity range, 20% of maximal activity at 0°C, high activity at 10-40°C | Deschampsia antarctica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
purified recombinant enzyme, 60 min, completely stable | Deschampsia antarctica |
| 90 | - |
purified recombinant enzyme, 60 min, loss of 20% activity | Deschampsia antarctica |
| 100 | - |
purified recombinant enzyme, 60 min, loss of 70% activity | Deschampsia antarctica |
| 110 | - |
purified recombinant enzyme, loss of 80% activity after 10 min, and inactivation after 20 min | Deschampsia antarctica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Deschampsia antarctica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | activity range | Deschampsia antarctica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme tertiary structural modeling | Deschampsia antarctica |
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