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Literature summary for 1.15.1.1 extracted from
- Molecular cloning, purification, and characterization of a superoxide dismutase from a fast-growing Mycobacterium sp. strain JC1 DSM 3803 (2011), J. Microbiol., 49, 399-406.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sodA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree | Mycobacterium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Mycobacterium sp. |  |
| additional information | poor inhibition by KCN, no inhibition by EDTA and cuprizone | Mycobacterium sp. | |
| NaN3 | slight inhibition | Mycobacterium sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mycobacterium sp. | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a cambialistic Fe/Mn-superoxide dismutase, 0.56 g-atom per mol of enzyme | Mycobacterium sp. | |
| Mn2+ | a cambialistic Fe/Mn-superoxide dismutase, 1.12 g-atom per mol of enzyme | Mycobacterium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22930 | - |
2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation | Mycobacterium sp. |
| 23000 | - |
2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation | Mycobacterium sp. |
| 48000 | - |
- |
Mycobacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium sp. | D0EAP3 | gene sodA | - |
| Mycobacterium sp. DSM 3803 | D0EAP3 | gene sodA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 115fold by heat treatment at 60°C for 10 min, gel filtration, anion exchange and hydrophobic interaction chromatography | Mycobacterium sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:methanol-grown cell | - |
Mycobacterium sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2524 | - |
purified native enzyme, pH 7.0, 25°C | Mycobacterium sp. |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation | Mycobacterium sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SOD | - |
Mycobacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | activity range, profile overview | Mycobacterium sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 50 | purified enzyme, 60 min, completely stable | Mycobacterium sp. |
| 60 | - |
purified enzyme, 60 min, loss of 20% activity | Mycobacterium sp. |
| 70 | - |
purified enzyme, 60 min, inactivation | Mycobacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Mycobacterium sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range, profile overview | Mycobacterium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified enzyme, 60 min, completely stable | Mycobacterium sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium sp. | sequence calculation | - |
5.33 |
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